三种不同产地决明子叶体外抗疟活性及毒性研究。

Q4 Medicine
African Journal of Infectious Diseases Pub Date : 2020-07-31 eCollection Date: 2020-01-01 DOI:10.21010/ajid.v14i2.4
Ezrani Tasiam, Riesta Primaharinastiti, Wiwied Ekasari
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引用次数: 2

摘要

背景:决明子叶的抗疟活性已被发现的活性化合物,即决明子素a所证实。众所周知,具有药用原料潜力的化合物的含量受多种因素的影响,包括植物来源的差异。本研究旨在比较Pariaman(海拔1000米)、Palu(海拔60米)和Surabaya(海拔2米)三个不同海拔位置值地区的沙麻叶的抗疟活性和毒性。材料和方法:本研究以Pariaman、Surabaya和Palu的Johar叶为原料,用正己烷和含有1%酒石酸的90%乙醇提取。采用恶性疟原虫3D7进行抗疟活性试验。毒性试验采用MTT ELISA法。结果:产自帕利曼的紫杉叶抗疟活性最高,IC50值为0.006μg/ml,产自帕卢的紫杉叶IC50值为0.037μg/ml,产自泗水的紫杉叶IC50值最低,为0.09μg/ml。CC50毒性测定结果显示,泗水地区毒性最高,CC50值为135.81μg/ml,帕鲁地区毒性最低,CC50值为220.82 μg/ml。结论:帕鲁地区所获得的沙麻叶具有一定的选择性,具有良好的抗疟作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<i>IN VITRO</i> ANTIMALARIAL ACTIVITY AND TOXICITY STUDIES OF JOHAR (<i>CASSIA SIAMEA</i>) LEAVES FROM THREE DIFFERENT LOCATIONS.

<i>IN VITRO</i> ANTIMALARIAL ACTIVITY AND TOXICITY STUDIES OF JOHAR (<i>CASSIA SIAMEA</i>) LEAVES FROM THREE DIFFERENT LOCATIONS.

<i>IN VITRO</i> ANTIMALARIAL ACTIVITY AND TOXICITY STUDIES OF JOHAR (<i>CASSIA SIAMEA</i>) LEAVES FROM THREE DIFFERENT LOCATIONS.

IN VITRO ANTIMALARIAL ACTIVITY AND TOXICITY STUDIES OF JOHAR (CASSIA SIAMEA) LEAVES FROM THREE DIFFERENT LOCATIONS.

Background: Antimalarial activity of Cassia siamea leaves has been proven by the active compound that has been found, i.e. Cassiarin A. It is known that the quantity of the content of a compound that has the potential as a raw material for medicine can be influenced by various factors including differences in plant origin. This study aims at comparing the antimalarial activity and toxicity of C.siamea leaves from three regions with different meters location values above sea level (asl), i.e Pariaman (1,000 m asl), Palu (60 m asl), and Surabaya (2 m asl).

Materials and methods: The materials used in this study were Johar leaves from Pariaman, Surabaya, and Palu extracted with n-hexane, and 90% ethanol containing 1% tartaric acid. The antimalarial activity test was done with Plasmodium falciparum 3D7. The toxicity test applied MTT ELISA method.

Results: C.siamea leaf that had highest antimalarial activity came from Pariaman with IC50 value of 0.006μg/ml, then from Palu was 0.037μg/ml, and the lowest antimalarial activity was from Surabaya that was 0.09μg/ml . In testing the toxicity to get CC50, the highest toxicity came from Surabaya with CC50 value of 135.81μg/ml, Pariaman with CC50 value of 220.82 μg/ml, and the least toxic came from Palu with CC50 value of 235.52μg/ml .

Conclusion: C.siamea leaf obtained from Pariaman had a selectivity index value that satisfies the requirements of a promising antimalarial effect.

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来源期刊
African Journal of Infectious Diseases
African Journal of Infectious Diseases Medicine-Infectious Diseases
CiteScore
1.60
自引率
0.00%
发文量
32
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