良性和恶性唾液腺肿瘤中细胞粘附分子的改变。β -整合素、粘粒素-2、ICAM-1和cd44的范例。

IF 1.9 3区 生物学 Q2 BIOLOGY
Dimitrios Andreadis, Athanasios Poulopoulos, Apostolos Epivatianos, Alexandros Nomikos, Dimitrios Parlitsis, Konstantinos Christidis, Calypso Barbatis, Dimitrios Kavvadas, Alexandros Toskas, Theodora Papamitsou, Dimitrios Antoniades
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引用次数: 2

摘要

背景:细胞间和细胞-细胞外基质连接的改变有助于肿瘤的发展。本研究探讨了特异性细胞粘附分子(CAMs)在唾液腺肿瘤(sgt)中的表达。方法:采用Envision/HRP免疫组化技术,对34例良、恶性SGTs和正常唾液腺不同类型组织标本进行福尔马林固定、石蜡包埋的检测,检测desmoglin -2 (Dsg-2)、β -整合素(β - 4-integrin)、CD44s和ICAM-1的表达。用半定量方法评价染色强度。使用Kendall’s τ和Spearman’s ρ作为相关标准对结果进行分析。结果:细胞间隙Dsg-2、细胞基底膜β -整合素、两种接触点CD44s均在正常腺泡细胞和导管细胞中强烈表达,而ICAM-1仅在内皮细胞和稀疏基质细胞及单核细胞中表达。Dsg-2在腺瘤和对照组以及腺癌和对照组中的表达有很强的相关性。在腺瘤中,除了常见的膜质表达外,还观察到明显的细胞质中存在Dsg-2,与正常组织相比表达减少。在恶性sgt中,Dsg-2不表达。在大多数sgt中,β -整合素也以一种独特的模式表达,涉及细胞质和非极化膜,而CD44仅在膜上发现。我们注意到β -整合素在腺瘤和对照组中的表达有很强的相关性,而CD44的表达在腺癌和对照组中也有显著的相关性(p结论:sgt中CAMs的不同表达谱可能提示了它们的发病机制,代表了肿瘤细胞如何利用正常组织结构和细胞外基质相互作用的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Cell adhesion molecules' altered profile in benign and malignant salivary gland tumors. The paradigm of beta4-integrin, desmoglein-2, ICAM-1 and CD44s.

Cell adhesion molecules' altered profile in benign and malignant salivary gland tumors. The paradigm of beta4-integrin, desmoglein-2, ICAM-1 and CD44s.

Cell adhesion molecules' altered profile in benign and malignant salivary gland tumors. The paradigm of beta4-integrin, desmoglein-2, ICAM-1 and CD44s.

Background: Alterations in intercellular and cell-extracellular matrix connections contribute to tumour development. This study investigates the expression of specific cell adhesion molecules (CAMs) in salivary gland tumors (SGTs).

Methods: Formalin-fixed, paraffin- embedded tissue specimens of different types of 34 benign and 31 malignant SGTs and normal salivary glands were studied using Envision/HRP immunohistochemical technique for Desmoglein-2 (Dsg-2), beta4-integrin, CD44s and ICAM-1. Intensity of staining was evaluated in a semi-quantitative manner. Results were analyzed using Kendall's τ and Spearman's ρ as correlation criteria.

Results: Dsg-2 in intercellular space, beta4-integrin in cell-basal membrane, and CD44s in both types of contacts were strongly expressed in normal acinar and ductal cells, whereas ICAM-1 was expressed only at the endothelium and sparse stromal cells and monocytes. Strong correlation was found between Dsg-2 expression in adenomas and controls and between adenocarcinomas and controls. In adenomas, a distinct cytoplasmic presence of Dsg-2 was observed in addition to the usual membranous expression, with decreased expression in comparison with normal tissue. In malignant SGTs, Dsg-2 expression was absent. In most SGTs, beta4-integrin was expressed also with a distinct pattern, involving the cytoplasm and the unpolarised membrane, while CD44 was found only on the membrane. Strong correlation between beta4-integrin expression in adenomas and controls was noted, while CD44 expression was found to be correlated significantly between adenocarcinomas and controls (p < 0.001). Regarding ICAM-1, its expression was found increased in adenomas, with non-specific distribution in malignant SGTs and strong correlation between the histological subtypes and controls (p < 0.001).

Conclusion: The different expression profile of CAMs in SGTs could possibly suggest a role on their pathogenesis, representing a model of how neoplastic cells can take advantage of normal tissue architecture and cell-extracellular matrix interactions.

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来源期刊
CiteScore
5.20
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.
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