神经营养因子-3对中枢听觉结构内禀神经元特性的影响。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2020-12-10 eCollection Date: 2020-01-01 DOI:10.1177/2633105520980442
Momoko Takahashi, Jason Tait Sanchez
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引用次数: 0

摘要

神经营养因子是一类控制神经元增殖、形态和凋亡的生长因子蛋白,在整个神经系统中无处不在。一种特殊的神经营养因子(NT-3)及其同源酪氨酸受体激酶(TrkC)最近受到关注,作为突触病变感音神经性听力损失的可能治疗靶点。此外,研究表明NT-3-TrkC信号在外周内耳耳蜗频率拓扑(即声位顺序)的感觉组织建立中起作用。然而,NT-3对中枢听觉特性的神经营养作用尚不清楚。在这项研究中,我们研究了NT-3-TrkC信号是否影响鸡的一级中央听觉结构,即大细胞核(NM)的固有电生理特性。在这里,特定神经营养因子的表达模式是众所周知的,并受到严格调控。通过使用全细胞膜片钳电生理学,我们发现将NT-3应用于脑干切片不会影响高频神经元区域的固有特性,但对低频神经元有强大的影响,改变电压依赖性钾功能、动作电位复极化动力学和被动膜特性。我们认为NT-3可能通过在调节低频NM神经元固有神经元特性的发展中发挥特殊作用,有助于中枢听觉通路中tontoptopy的精确建立和组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure.

Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure.

Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure.

Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure.

Neurotrophins, a class of growth factor proteins that control neuronal proliferation, morphology, and apoptosis, are found ubiquitously throughout the nervous system. One particular neurotrophin (NT-3) and its cognate tyrosine receptor kinase (TrkC) have recently received attention as a possible therapeutic target for synaptopathic sensorineural hearing loss. Additionally, research shows that NT-3-TrkC signaling plays a role in establishing the sensory organization of frequency topology (ie, tonotopic order) in the cochlea of the peripheral inner ear. However, the neurotrophic effects of NT-3 on central auditory properties are unclear. In this study we examined whether NT-3-TrkC signaling affects the intrinsic electrophysiological properties at a first-order central auditory structure in chicken, known as nucleus magnocellularis (NM). Here, the expression pattern of specific neurotrophins is well known and tightly regulated. By using whole-cell patch-clamp electrophysiology, we show that NT-3 application to brainstem slices does not affect intrinsic properties of high-frequency neuronal regions but had robust effects for low-frequency neurons, altering voltage-dependent potassium functions, action potential repolarization kinetics, and passive membrane properties. We suggest that NT-3 may contribute to the precise establishment and organization of tonotopy in the central auditory pathway by playing a specialized role in regulating the development of intrinsic neuronal properties of low-frequency NM neurons.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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