细胞培养塑料器皿对人细胞因子诱导杀伤细胞培养效果的比较。

IF 2.3 4区医学 Q3 ALLERGY

Asian Pacific journal of allergy and immunology Pub Date : 2023-12-01 DOI:10.12932/AP-140720-0913

Panwadee Pluangnooch, Chaiphichit Phayangkhe, Kitipong Soontrapa

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引用次数: 0

摘要

背景:细胞因子诱导的杀伤细胞(CIK)是体外扩增的T细胞，具有T细胞和自然杀伤细胞的表型。目的:比较三种细胞培养塑料器皿培养人CIK细胞的增殖和功能特性。方法:监测CIK细胞的数量和活力。流式细胞术检测细胞表面标志物(CD3和CD56)、TH1细胞因子(IFN-γ和TNF-α)和细胞溶解颗粒(颗粒酶B和穿孔素)的表达。结果:与培养皿和透气瓶培养的CIK细胞相比，静袋培养的CIK细胞数量最多。然而，在所有塑料器皿中培养的CIK细胞都表达表面标记物、TH1细胞因子和细胞溶解颗粒。结论:综合考虑安全性、有效性和成本，静态袋是培养CIK细胞的最佳塑料器皿。

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A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture.

Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties.

Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares.

Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry.

Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules.

Conclusions: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.

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来源期刊

Asian Pacific journal of allergy and immunology 医学-过敏

CiteScore

12.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.