Zafer Sahin, Alpaslan Ozkurkculer, Omer Faruk Kalkan, Ahmet Ozkaya, Aynur Koc, Raviye Ozen Koca, Hatice Solak, Zulfikare Isik Solak Gormus, Selim Kutlu
{"title":"慢性固定应激诱导雄性大鼠焦虑相关行为并影响脑必需矿物质。","authors":"Zafer Sahin, Alpaslan Ozkurkculer, Omer Faruk Kalkan, Ahmet Ozkaya, Aynur Koc, Raviye Ozen Koca, Hatice Solak, Zulfikare Isik Solak Gormus, Selim Kutlu","doi":"10.1024/0300-9831/a000682","DOIUrl":null,"url":null,"abstract":"<p><p><b></b> Alterations of essential elements in the brain are associated with the pathophysiology of many neuropsychiatric disorders. It is known that chronic/overwhelming stress may cause some anxiety and/or depression. We aimed to investigate the effects of two different chronic immobilization stress protocols on anxiety-related behaviors and brain minerals. Adult male Wistar rats were divided into 3 groups as follows (<i>n</i> = 10/group): control, immobilization stress-1 (45 minutes daily for 7-day) and immobilization stress-2 (45 minutes twice a day for 7-day). Stress-related behaviors were evaluated by open field test and forced swimming test. In the immobilization stress-1 and immobilization stress-2 groups, percentage of time spent in the central area (6.38 ± 0.41% and 6.28 ± 1.03% respectively, <i>p</i> < 0.05) and rearing frequency (2.75 ± 0.41 and 3.85 ± 0.46, <i>p</i> < 0.01 and <i>p</i> < 0.05, respectively) were lower, latency to center area (49.11 ± 5.87 s and 44.92 ± 8.04 s, <i>p</i> < 0.01 and <i>p</i> < 0.01, respectively), were higher than the control group (8.65 ± 0.49%, 5.37 ± 0.44 and 15.3 ± 3.32 s, respectively). In the immobilization stress-1 group, zinc (12.65 ± 0.1 ppm, <i>p</i> < 0.001), magnesium (170.4 ± 1.7 ppm, <i>p</i> < 0.005) and phosphate (2.76 ± 0.1 ppm, <i>p</i> < 0.05) levels were lower than the control group (13.87 ± 0.16 ppm, 179.31 ± 1.87 ppm and 3.11 ± 0.06 ppm, respectively). In the immobilization stress-2 group, magnesium (171.56 ± 1.87 ppm, <i>p</i> < 0.05), phosphate (2.44 ± 0.07 ppm, <i>p</i> < 0.001) levels were lower, and manganese (373.68 ± 5.76 ppb, <i>p</i> < 0.001) and copper (2.79 ± 0.15 ppm, <i>p</i> < 0.05) levels were higher than the control group (179.31 ± 1.87 ppm, 3.11 ± 0.06 ppm, 327.25 ± 8.35 ppb and 2.45 ± 0.05 ppm, respectively). Our results indicated that 7-day chronic immobilization stress increased anxiety-related behaviors in both stress groups. Zinc, magnesium, phosphate, copper and manganese levels were affected in the brain.</p>","PeriodicalId":13884,"journal":{"name":"International Journal for Vitamin and Nutrition Research","volume":"92 5-6","pages":"349-356"},"PeriodicalIF":2.0000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Chronic immobilization stress induces anxiety-related behaviors and affects brain essential minerals in male rats.\",\"authors\":\"Zafer Sahin, Alpaslan Ozkurkculer, Omer Faruk Kalkan, Ahmet Ozkaya, Aynur Koc, Raviye Ozen Koca, Hatice Solak, Zulfikare Isik Solak Gormus, Selim Kutlu\",\"doi\":\"10.1024/0300-9831/a000682\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b></b> Alterations of essential elements in the brain are associated with the pathophysiology of many neuropsychiatric disorders. It is known that chronic/overwhelming stress may cause some anxiety and/or depression. We aimed to investigate the effects of two different chronic immobilization stress protocols on anxiety-related behaviors and brain minerals. Adult male Wistar rats were divided into 3 groups as follows (<i>n</i> = 10/group): control, immobilization stress-1 (45 minutes daily for 7-day) and immobilization stress-2 (45 minutes twice a day for 7-day). Stress-related behaviors were evaluated by open field test and forced swimming test. In the immobilization stress-1 and immobilization stress-2 groups, percentage of time spent in the central area (6.38 ± 0.41% and 6.28 ± 1.03% respectively, <i>p</i> < 0.05) and rearing frequency (2.75 ± 0.41 and 3.85 ± 0.46, <i>p</i> < 0.01 and <i>p</i> < 0.05, respectively) were lower, latency to center area (49.11 ± 5.87 s and 44.92 ± 8.04 s, <i>p</i> < 0.01 and <i>p</i> < 0.01, respectively), were higher than the control group (8.65 ± 0.49%, 5.37 ± 0.44 and 15.3 ± 3.32 s, respectively). In the immobilization stress-1 group, zinc (12.65 ± 0.1 ppm, <i>p</i> < 0.001), magnesium (170.4 ± 1.7 ppm, <i>p</i> < 0.005) and phosphate (2.76 ± 0.1 ppm, <i>p</i> < 0.05) levels were lower than the control group (13.87 ± 0.16 ppm, 179.31 ± 1.87 ppm and 3.11 ± 0.06 ppm, respectively). In the immobilization stress-2 group, magnesium (171.56 ± 1.87 ppm, <i>p</i> < 0.05), phosphate (2.44 ± 0.07 ppm, <i>p</i> < 0.001) levels were lower, and manganese (373.68 ± 5.76 ppb, <i>p</i> < 0.001) and copper (2.79 ± 0.15 ppm, <i>p</i> < 0.05) levels were higher than the control group (179.31 ± 1.87 ppm, 3.11 ± 0.06 ppm, 327.25 ± 8.35 ppb and 2.45 ± 0.05 ppm, respectively). Our results indicated that 7-day chronic immobilization stress increased anxiety-related behaviors in both stress groups. Zinc, magnesium, phosphate, copper and manganese levels were affected in the brain.</p>\",\"PeriodicalId\":13884,\"journal\":{\"name\":\"International Journal for Vitamin and Nutrition Research\",\"volume\":\"92 5-6\",\"pages\":\"349-356\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2022-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal for Vitamin and Nutrition Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1024/0300-9831/a000682\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/9/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal for Vitamin and Nutrition Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1024/0300-9831/a000682","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/9/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 3
摘要
大脑中基本元素的改变与许多神经精神疾病的病理生理有关。众所周知,长期的/压倒性的压力可能会导致一些焦虑和/或抑郁。我们的目的是研究两种不同的慢性固定应激方案对焦虑相关行为和脑矿物质的影响。将成年雄性Wistar大鼠分为3组(n = 10/组):对照组、固定应激-1组(每天45分钟,连续7 d)和固定应激-2组(45分钟,每天2次,连续7 d)。采用野外试验和强迫游泳试验评价应激相关行为。在固定应激1组和固定应激2组中,在中心区停留的时间百分比分别为6.38±0.41%和6.28±1.03%,p p p p p p p p p p p p p p p p p p p p
Chronic immobilization stress induces anxiety-related behaviors and affects brain essential minerals in male rats.
Alterations of essential elements in the brain are associated with the pathophysiology of many neuropsychiatric disorders. It is known that chronic/overwhelming stress may cause some anxiety and/or depression. We aimed to investigate the effects of two different chronic immobilization stress protocols on anxiety-related behaviors and brain minerals. Adult male Wistar rats were divided into 3 groups as follows (n = 10/group): control, immobilization stress-1 (45 minutes daily for 7-day) and immobilization stress-2 (45 minutes twice a day for 7-day). Stress-related behaviors were evaluated by open field test and forced swimming test. In the immobilization stress-1 and immobilization stress-2 groups, percentage of time spent in the central area (6.38 ± 0.41% and 6.28 ± 1.03% respectively, p < 0.05) and rearing frequency (2.75 ± 0.41 and 3.85 ± 0.46, p < 0.01 and p < 0.05, respectively) were lower, latency to center area (49.11 ± 5.87 s and 44.92 ± 8.04 s, p < 0.01 and p < 0.01, respectively), were higher than the control group (8.65 ± 0.49%, 5.37 ± 0.44 and 15.3 ± 3.32 s, respectively). In the immobilization stress-1 group, zinc (12.65 ± 0.1 ppm, p < 0.001), magnesium (170.4 ± 1.7 ppm, p < 0.005) and phosphate (2.76 ± 0.1 ppm, p < 0.05) levels were lower than the control group (13.87 ± 0.16 ppm, 179.31 ± 1.87 ppm and 3.11 ± 0.06 ppm, respectively). In the immobilization stress-2 group, magnesium (171.56 ± 1.87 ppm, p < 0.05), phosphate (2.44 ± 0.07 ppm, p < 0.001) levels were lower, and manganese (373.68 ± 5.76 ppb, p < 0.001) and copper (2.79 ± 0.15 ppm, p < 0.05) levels were higher than the control group (179.31 ± 1.87 ppm, 3.11 ± 0.06 ppm, 327.25 ± 8.35 ppb and 2.45 ± 0.05 ppm, respectively). Our results indicated that 7-day chronic immobilization stress increased anxiety-related behaviors in both stress groups. Zinc, magnesium, phosphate, copper and manganese levels were affected in the brain.
期刊介绍:
Since 1930 this journal has provided an important international forum for scientific advances in the study of nutrition and vitamins. Widely read by academicians as well as scientists working in major governmental and corporate laboratories throughout the world, this publication presents work dealing with basic as well as applied topics in the field of micronutrients, macronutrients, and non-nutrients such as secondary plant compounds.
The editorial and advisory boards include many of the leading persons currently working in this area.
The journal is of particular interest to:
- Nutritionists
- Vitaminologists
- Biochemists
- Physicians
- Engineers of human and animal nutrition
- Food scientists