家蚕细胞系中的持续性病毒:家蚕潜伏病毒。

Uirusu Pub Date : 2019-01-01 DOI:10.2222/jsv.68.137
Masashi Iwanaga
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引用次数: 2

摘要

家蚕潜伏病毒(Bombyx mori latent virus, BmLV)是一种单链阳性昆虫RNA病毒,与植物tymovirus关系密切,目前被归类为“未分类”tymovirus。BmLV仅在家蚕(Bombyx mori)的细胞系中以极高的水平积累,但它不会导致致命并造成持续感染。目前尚不清楚BmLV是否影响家蚕核多角体病毒杆状病毒表达载体系统,以及BmLV如何在昆虫细胞系中复制和建立持续感染。本文就BmLV的发现、无病毒培养细胞的建立以及该病毒的安全性作一综述。我还描述了两种不同的小rna介导途径维持bmlv感染细胞中的病毒水平,从而使病毒建立持续感染。病毒衍生的小干扰rna (vsirna)和piwi相互作用rna (vpiRNAs)都是随着BmLV感染的进展而产生的。我们发现siRNA途径在BmLV的急性和持续感染中都起作用,而piRNA途径仅在该病毒的持续感染中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Persistent virus in the silkworm cell lines: Bombyx mori latent virus].

Bombyx mori latent virus (BmLV) is a positive, single-stranded insect RNA virus with a close relationship to plant tymoviruses and currently classified as an "unclassified" tymovirus. BmLV is accumulated at extremely high levels only in cell lines derived from the silkworm, Bombyx mori, but it does not lead to lethality and establishes persistent infections. It was unknown whether BmLV affects the Baculovirus Expression Vector System using Bombyx mori nucleopolyhedrovirus, and how BmLV replicates and establishes persistent infections in insect cell lines. In this review, I introduce the discovery of BmLV, the establishment of virus-free cultured cells and the safety aspect of this virus. I also describe that two distinct small RNA-mediated pathways maintain the virus level in BmLV-infected cells, thereby allowing the virus to establish persistent infection. Virus-derived small interfering RNAs (vsiRNAs) and PIWI-interacting RNAs (vpiRNAs) are both produced as the BmLV infection progressed. We revealed that while siRNA pathway functions in both acute and persistent infection of BmLV, piRNA pathway functions only in the persistent infection of this virus.

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