白鲟鱼种链球菌病的腹腔和肌肉注射攻毒模型

IF 1.5 4区 农林科学 Q3 FISHERIES
Diem Thu Nguyen, David Marancik, Esteban Soto
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引用次数: 2

摘要

牛链球菌是一种人畜共患病原体,是引起链球菌病的主要病原之一。在跨山白鲟中,猪链球菌感染通常表现为坏死性和嗜异性性肌炎,在受感染的鱼中造成30-50%的死亡率。为了更好地了解白鲟链球菌病的发病机制,并确定最接近自然疾病的实验感染途径,通过腔内/腹腔(IC)或肌内(IM)途径,对鱼种进行单剂量1.3 × 108个细胞/条的猪链球菌感染。急性死亡仅出现在im攻击鱼中,第一次死亡发生在攻击后4天,第9天死亡率达到18.3%。攻击鱼表现为游动不稳定,皮肤溃疡性病变,肝脏和鱼鳔出血。从死鱼和死鱼的肾脏和脑组织中检出牛链球菌。对急性死亡的鱼的组织病理学分析显示,注射部位的肌肉和血管器官(如心脏和脾脏)内的细菌大量增殖,并伴有不同程度的组织坏死,包括坏死性肌炎。攻击后近9天死亡的鱼在注射部位、肝脏、肾脏和脾脏的骨骼肌表现出更明显的多灶性局部广泛肉芽肿炎症。在对照组或ic挑战鱼中未观察到死亡、临床症状或明显变化。对每组治疗的10名幸存者进行死后评估,以确定脑和后肾组织中的携带者状态。在IM和ic挑战组中,存活者中链球菌的患病率分别为10%和0%。本研究结果提示im注射攻毒法适用于白鲟链球菌病的诱导,可能是研究该物种自然发生疾病发病机制的首选方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intracoelomic- and Intramuscular-Injection Challenge Model of Piscine Streptococcosis in White Sturgeon Fingerlings

Streptococcus iniae is a zoonotic pathogen and one of the major aetiologic agents of streptococcosis. In White Sturgeon Acipenser transmontanus, S. iniae infection typically presents as a necrotizing and heterophilic myositis, causing 30–50% mortality in infected fish. To gain a better understanding of the pathogenesis of streptococcosis in White Sturgeon, and to identify the experimental route of infection that most closely mimics the natural disease, fingerlings were challenged with a single dose of 1.3 × 108 cells/fish of S. iniae that was administered via intracoelomic/intraperitoneal (IC) or intramuscular (IM) routes. Acute mortalities were present only in the IM-challenged fish, with first mortality occurring 4 d postchallenge and the mortality rate reaching 18.3% after 9 d. The challenged fish presented erratic swimming, ulcerative skin lesions, and hemorrhages in the liver and swim bladder. Streptococcus iniae was recovered from the kidney and brain tissues of moribund and dead fish. Histopathologic analysis of fish that died acutely revealed massive proliferation of bacteria in the muscle at the injection site and within vascular organs such as the heart and spleen, with variable amounts of tissue necrosis including a necrotizing myositis. Fish that died closer to 9 d postchallenge demonstrated more pronounced multifocal to locally extensive granulomatous inflammation of skeletal muscle at the injection site, liver, kidney, and spleen. No mortality, clinical signs, or gross changes were observed in the control or IC-challenged fish. Postmortem evaluation of 10 survivors in each treatment was performed to determine carrier status in the brain and posterior kidney tissues. The prevalence of S. iniae in survivors was 10% and 0% in the IM- and IC-challenged groups, respectively. The results from this study suggest that IM-injection challenge methods are suitable for inducing streptococcosis in White Sturgeon, and they may be the preferred method for studying the pathogenesis of the naturally occurring disease in this species.

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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