足菌肿骨组织常规脱钙与微波辅助脱钙的比较。

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2020-08-01 eCollection Date: 2020-01-01 DOI:10.1155/2020/6561980
Magdi Mansour Salih
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引用次数: 4

摘要

足菌肿是一种皮下组织和骨骼的终身肉芽肿性疾病。组织病理学是一种有根据的指示性方法,基于对足菌肿的明确诊断的假设。它需要有效地处理组织,包括骨脱钙。脱钙过程必须确保钙的完全去除,并适当地保存组织和微生物的染色能力。目标。采用不同的脱钙溶液,对传统脱钙方法与微波脱钙方法进行比较。测试了不同的特征,包括脱钙速度,形态学和真菌保存在受足菌肿影响的骨组织中。材料与方法。采用三种脱钙溶液,包括10%中性缓冲EDTA (pH 7.4)、5%硝酸和5%盐酸,从50个足菌肿骨组织样本中去除钙。采用常规法和微波法。采用血红素-伊红(HE)染色、Gridley染色和Grocott六检银染色评价骨和真菌形态。结果。与添加10% EDTA (pH 7.4)的微波法相比,常规法脱钙时间分别为120小时和29小时,5%盐酸和5%硝酸脱钙时间分别为8小时和3小时。另外,10% EDTA是HE染色和真菌染色的最佳脱钙剂。5%盐酸和5%硝酸可用于真菌染色。结论。本研究探讨了不同的脱钙剂以及两种脱钙方法对骨结构保存和真菌染色的影响,这将有助于制定合适的方案来分析受足菌肿感染的骨组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma.

Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma.

Mycetoma is a lifelong granulomatous disease of subcutaneous tissues and bones. Histopathology is a substantiated indicative method based on the assumption of a definitive diagnosis of mycetoma. It requires efficient processing of tissues including bone decalcification. The decalcification process must ensure complete removal of calcium and also a proper preservation of tissue and microorganisms' staining ability. Objectives. To compare the conventional method used in decalcification with the microwave method using different decalcification solutions. Different characteristics were tested, including the speed of decalcification and morphological and fungal preservation in bone tissue affected with mycetoma. Materials and Methods. Three decalcification solutions were employed to remove calcium from 50 bone tissue samples affected with mycetoma, including 10% neutral buffered EDTA (pH 7.4), 5% nitric acid, and 5% hydrochloric acid. Conventional and microwave methods were used. Haematoxylin-eosin (HE) stain, Gridley's stain, and Grocott hexamine-silver stain were employed to evaluate the bone and fungi morphologies. Results. The decalcification time of the conventional method compared with the microwave method with 10% EDTA (pH 7.4) took 120 hours and 29 hours, while 5% hydrochloric acid and 5% nitric acid took 8 hours and 3 hours, separately. Also, 10% EDTA is the best decalcifying agent for HE staining and fungal stains. 5% hydrochloric acid and 5% nitric acid can be used for fungal staining. Conclusion. The current study investigated the effects of different decalcifying agents as well as two decalcification procedures on the preservation of the bone structure and fungal staining, which will help to develop suitable protocols for the analyses of the bone tissue affected with mycetoma infection.

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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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