DPPD对氯化汞所致大鼠肝肾毒性的保护作用。

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2020-07-15 eCollection Date: 2020-01-01 DOI:10.1155/2020/4127284
Ahmed Nabil, Mohamed M Elshemy, Medhat Asem, Heba F Gomaa
{"title":"DPPD对氯化汞所致大鼠肝肾毒性的保护作用。","authors":"Ahmed Nabil,&nbsp;Mohamed M Elshemy,&nbsp;Medhat Asem,&nbsp;Heba F Gomaa","doi":"10.1155/2020/4127284","DOIUrl":null,"url":null,"abstract":"<p><p>Mercury is a global environmental pollutant, accumulating mainly in the kidney and liver inducing hepatorenal toxicity, oxidative stress, and tissue damage. Oxidative stress is caused by an imbalance between free radicals' production and cellular antioxidant defense systems. In the present study, we investigated the effect of N N'-diphenyl-1, 4-phenylenediamine (DPPD) antioxidant activity against mercury chloride- (HgCl<sub>2</sub>-) induced renal and hepatic toxicity. Thirty adult female Sprague Dawley rats were divided into three equal groups: the first group was injected with saline only and served as a control, the second group was injected with HgCl<sub>2</sub>, and the third group received DPPD + HgCl<sub>2</sub> rats injected with HgCl<sub>2</sub> without treatment showing a significant increase in alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, and uric acids compared to control. Moreover, the second group showed a significant reduction in the activity of the antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH)) in addition to a marked increase in the malondialdehyde (MDA) content, histopathological alterations, collagen deposition, CD8%, CD4%, and TGF-<i>β</i>% in kidney and liver tissues compared with the control group. Treatment with DPPD showed significant recovery (<i>p</i> ≤ 0.001) in all previous parameters and histopathological examination. In conclusion, we suggested that DPPD may have a promising antioxidant capacity, gives it the applicability to be used as a prophylactic agent against mercury-induced hepatorenal cytotoxicity in the future.</p>","PeriodicalId":17421,"journal":{"name":"Journal of Toxicology","volume":"2020 ","pages":"4127284"},"PeriodicalIF":3.4000,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/4127284","citationCount":"10","resultStr":"{\"title\":\"Protective Effect of DPPD on Mercury Chloride-Induced Hepatorenal Toxicity in Rats.\",\"authors\":\"Ahmed Nabil,&nbsp;Mohamed M Elshemy,&nbsp;Medhat Asem,&nbsp;Heba F Gomaa\",\"doi\":\"10.1155/2020/4127284\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mercury is a global environmental pollutant, accumulating mainly in the kidney and liver inducing hepatorenal toxicity, oxidative stress, and tissue damage. Oxidative stress is caused by an imbalance between free radicals' production and cellular antioxidant defense systems. In the present study, we investigated the effect of N N'-diphenyl-1, 4-phenylenediamine (DPPD) antioxidant activity against mercury chloride- (HgCl<sub>2</sub>-) induced renal and hepatic toxicity. Thirty adult female Sprague Dawley rats were divided into three equal groups: the first group was injected with saline only and served as a control, the second group was injected with HgCl<sub>2</sub>, and the third group received DPPD + HgCl<sub>2</sub> rats injected with HgCl<sub>2</sub> without treatment showing a significant increase in alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, and uric acids compared to control. Moreover, the second group showed a significant reduction in the activity of the antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH)) in addition to a marked increase in the malondialdehyde (MDA) content, histopathological alterations, collagen deposition, CD8%, CD4%, and TGF-<i>β</i>% in kidney and liver tissues compared with the control group. Treatment with DPPD showed significant recovery (<i>p</i> ≤ 0.001) in all previous parameters and histopathological examination. In conclusion, we suggested that DPPD may have a promising antioxidant capacity, gives it the applicability to be used as a prophylactic agent against mercury-induced hepatorenal cytotoxicity in the future.</p>\",\"PeriodicalId\":17421,\"journal\":{\"name\":\"Journal of Toxicology\",\"volume\":\"2020 \",\"pages\":\"4127284\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2020-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2020/4127284\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1155/2020/4127284\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2020/4127284","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 10

摘要

汞是一种全球性的环境污染物,主要积聚在肾脏和肝脏中,引起肝肾毒性、氧化应激和组织损伤。氧化应激是由自由基产生和细胞抗氧化防御系统之间的不平衡引起的。在本研究中,我们研究了N N'-二苯基- 1,4 -苯二胺(DPPD)抗氯化汞(HgCl2-)诱导的肾和肝毒性的作用。将30只成年雌性Sprague Dawley大鼠分为三组,第一组只注射生理盐水作为对照,第二组注射HgCl2,第三组注射DPPD + HgCl2,未经处理,注射HgCl2的大鼠碱性磷酸酶(ALP)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、尿素、肌酐和尿酸均较对照组显著升高。此外,与对照组相比,第二组抗氧化酶(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH))活性显著降低,肾脏和肝脏组织丙二醛(MDA)含量、组织病理学改变、胶原沉积、CD8%、CD4%和TGF-β%明显增加。经DPPD治疗后,术前各项指标及组织病理学检查均有显著恢复(p≤0.001)。综上所述,我们认为DPPD可能具有良好的抗氧化能力,使其在未来可作为汞诱导的肝肾细胞毒性的预防剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protective Effect of DPPD on Mercury Chloride-Induced Hepatorenal Toxicity in Rats.

Protective Effect of DPPD on Mercury Chloride-Induced Hepatorenal Toxicity in Rats.

Protective Effect of DPPD on Mercury Chloride-Induced Hepatorenal Toxicity in Rats.

Mercury is a global environmental pollutant, accumulating mainly in the kidney and liver inducing hepatorenal toxicity, oxidative stress, and tissue damage. Oxidative stress is caused by an imbalance between free radicals' production and cellular antioxidant defense systems. In the present study, we investigated the effect of N N'-diphenyl-1, 4-phenylenediamine (DPPD) antioxidant activity against mercury chloride- (HgCl2-) induced renal and hepatic toxicity. Thirty adult female Sprague Dawley rats were divided into three equal groups: the first group was injected with saline only and served as a control, the second group was injected with HgCl2, and the third group received DPPD + HgCl2 rats injected with HgCl2 without treatment showing a significant increase in alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, and uric acids compared to control. Moreover, the second group showed a significant reduction in the activity of the antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH)) in addition to a marked increase in the malondialdehyde (MDA) content, histopathological alterations, collagen deposition, CD8%, CD4%, and TGF-β% in kidney and liver tissues compared with the control group. Treatment with DPPD showed significant recovery (p ≤ 0.001) in all previous parameters and histopathological examination. In conclusion, we suggested that DPPD may have a promising antioxidant capacity, gives it the applicability to be used as a prophylactic agent against mercury-induced hepatorenal cytotoxicity in the future.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信