"One-gate" design should be used for the study of the diagnostic efficacy of biological markers.

1. The first draft of this article proposes "Please authors answer a question: was there only 43 patients with diagnosed gastric cancer from April 2018 to July 2018?" Is it to know whether the researchers chose 43 patients because of fewer cases or intentionally fewer? The reviewer's "response reviewers" admitted "it's better to add a benign disease group" and "chose 43 patients with diagnosed gastric cancer randomly for our validation," which confirmed my opinion "have design flaws both in enroll subjects and the number of cases". So after getting the reply from the original author, I deleted the above sentence. This time amend "43 patients" on line 43 to "43 patients which random selection rather than all patients in the study time window". 2. Some revision has been highlighted in red. 3. We re-edit the article sentence by sentence in English to make it more in line with the English expression.