意大利犬MDR1基因的基因型和等位基因频率。

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2020-06-24 eCollection Date: 2020-01-01 DOI:10.1136/vetreco-2019-000375
Stefano Paolo Marelli, Michele Polli, Stefano Frattini, Matteo Cortellari, Rita Rizzi, Paola Crepaldi
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引用次数: 5

摘要

背景:犬多药耐药基因MDR1(也称为ABCB1)的突变,编码多药耐药(MDR) p糖蛋白(P-gp)应答器,导致称为“伊维菌素中毒”的病理状态。自2001年以来已知的致病突变已被描述为影响与牧羊犬血统相关的绵羊放牧品种。本研究对意大利犬种群中MDR1突变等位基因的存在进行了5年的回顾性调查。该研究的目的是深入了解意大利犬种和犬群的MDR1等位基因和基因型频率。方法:收集32个犬种/群体811只犬MDR1基因4 bp缺失(c296_299del4)的基因型数据。结果:在31个品种中的9个品种中发现了突变等位基因:粗毛牧羊犬,光滑牧羊犬,边境牧羊犬,胡须牧羊犬,设得兰牧羊犬,澳大利亚牧羊犬,白瑞士牧羊犬,古英国牧羊犬,惠比特犬和杂交犬。等位基因突变频率最高的品种是光滑牧羊犬和粗牧羊犬,分别有75%和66%的突变MDR1等位基因。结论:结果支持该遗传分析的有效性,以优化具有多药耐药风险的犬的医疗护理,并为育种计划定义和不同品种的风险评估提供客观基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genotypic and allelic frequencies of <i>MDR1</i> gene in dogs in Italy.

Genotypic and allelic frequencies of MDR1 gene in dogs in Italy.

Background: A mutation in the canine multidrug resistance MDR1 gene (also referred as ABCB1), encoding for the multidrug resistance (MDR) P-glycoprotein (P-gp) transponder, causes a pathological condition known as 'ivermectin toxicosis'. The causative mutation, known since 2001, has been described to affects sheep herding breeds related to collie lineage. The present study is a retrospective investigation of the presence of MDR1 mutated allele in Italian dog populations in a 5 years' time lapse. The aim of the research is to offer a deep knowledge in MDR1 allelic and genotypic frequencies in canine breeds and populations raised in Italy.

Methods: Genotype data for the 4-bp deletion (c296_299del4) in MDR1 gene from 811 dogs belonging to 32 breeds/populations were collected.

Results: The mutated allele has been found in 9 out of 31 breeds: Rough Collie, Smooth Collie, Border Collie, Bearded Collie, Shetland Sheepdog, Australian Shepherd, White Swiss Shepherd, Old English Sheepdog, Whippet and also in crossbreed. The breeds with the highest allelic mutation frequency are Smooth and Rough Collies with 75 per cent and 66 per cent of mutant MDR1 allele, respectively.

Conclusions: The results support the usefulness of this genetic analysis to optimise medical care in dogs at risk of multidrug resistance and to create an objective basis in breeding programme definition and in the risk evaluation in different breeds.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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