用于丝状真菌天然产物发现和工程的不断扩大的CRISPR工具箱†

IF 10.6 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Natural Product Reports Pub Date : 2023-01-01 DOI:10.1039/d2np00055e

Clara Woodcraft , Yit-Heng Chooi , Indra Roux

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引用次数: 4

摘要

报道：截至2022年5月，真菌遗传学通过阐明神秘代谢物和生物合成步骤，改变了天然产物研究。通过CRISPR/Cas技术增加、减少、调节和重写基因的能力增强，为在不同丝状真菌中操纵生物合成基因簇开辟了途径。这篇综述讨论了基于CRISPR/Cas的工具使真菌天然产物的发现和工程成为可能的创新和多样化的策略。我们还从多个角度为CRISPR/Cas实验设计提供了指导，并讨论了丝状真菌遗传工具开发的当前差距以及天然产物研究的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi†

查看原文本刊更多论文

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi†

Covering: up to May 2022

Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

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来源期刊

Natural Product Reports 化学-生化与分子生物学

CiteScore

21.20

自引率

3.40%

发文量

127

审稿时长

1.7 months

期刊介绍： Natural Product Reports (NPR) serves as a pivotal critical review journal propelling advancements in all facets of natural products research, encompassing isolation, structural and stereochemical determination, biosynthesis, biological activity, and synthesis. With a broad scope, NPR extends its influence into the wider bioinorganic, bioorganic, and chemical biology communities. Covering areas such as enzymology, nucleic acids, genetics, chemical ecology, carbohydrates, primary and secondary metabolism, and analytical techniques, the journal provides insightful articles focusing on key developments shaping the field, rather than offering exhaustive overviews of all results. NPR encourages authors to infuse their perspectives on developments, trends, and future directions, fostering a dynamic exchange of ideas within the natural products research community.