高尔基体α1,2-甘露糖苷酶 I 在上皮细胞迁移过程中诱导 CD147 的聚集和分区。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Miguel Gonzalez-Andrades, Supriya S Jalimarada, Maria Rodriguez-Benavente, Marissa N Feeley, Ashley M Woodward, Dina B AbuSamra, Pablo Argüeso
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引用次数: 0

摘要

CD147 是一种广泛表达的基质金属蛋白酶诱导剂,参与细胞迁移的调控。CD147的高糖基化和寡聚化能力与CD147的功能有关,但人们对这些过程背后的分子机制了解有限。目前的研究表明,在细胞迁移过程中,高尔基体α1,2-甘露糖苷酶I的表达是维持CD147细胞表面组织的关键。利用分层人角膜上皮伤口愈合的体外模型,我们发现 CD147 聚集在相邻迁移细胞前缘的侧浆膜内。这种定位与基质金属蛋白酶活性的激增和α1,2-甘露糖苷酶亚型 IC(MAN1C1)表达的增加相关。用脱氧野尻霉素全面抑制α1,2-甘露糖苷酶 I 的活性,可明显减弱 CD147 的糖基化并破坏其在前缘的表面分布,同时降低基质金属蛋白酶-9 的表达。同样,脱氧甘露尻霉素或 siRNA 介导的 MAN1C1 基因敲除也会削弱碳水化合物结合蛋白 galectin-3 在未损伤细胞中刺激 CD147 聚集的能力。我们的结论是,α1,2-甘露糖苷酶 I 的甘露糖修饰活性协调了上皮损伤后 CD147 的聚集和分区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.

Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.

Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.

Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.

CD147 is a widely expressed matrix metalloproteinase inducer involved in the regulation of cell migration. The high glycosylation and ability to undergo oligomerization have been linked to CD147 function, yet there is limited understanding on the molecular mechanisms behind these processes. The current study demonstrates that the expression of Golgi α1,2-mannosidase I is key to maintaining the cell surface organization of CD147 during cell migration. Using an in vitro model of stratified human corneal epithelial wound healing, we show that CD147 is clustered within lateral plasma membranes at the leading edge of adjacent migrating cells. This localization correlates with a surge in matrix metalloproteinase activity and an increase in the expression of α1,2-mannosidase subtype IC (MAN1C1). Global inhibition of α1,2-mannosidase I activity with deoxymannojirimycin markedly attenuates the glycosylation of CD147 and disrupts its surface distribution at the leading edge, concomitantly reducing the expression of matrix metalloproteinase-9. Likewise, treatment with deoxymannojirimycin or siRNA-mediated knockdown of MAN1C1 impairs the ability of the carbohydrate-binding protein galectin-3 to stimulate CD147 clustering in unwounded cells. We conclude that the mannose-trimming activity of α1,2-mannosidase I coordinates the clustering and compartmentalization of CD147 that follows an epithelial injury.

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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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