利用单细胞和空间转录组了解胚胎早期发育过程中的干细胞谱系特征。

IF 7.7 2区 生物学 Q1 GENETICS & HEREDITY
Guangdun Peng, Guizhong Cui, Jincan Ke, Naihe Jing
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引用次数: 26

摘要

胚胎发育和干细胞分化为理解协调的细胞命运决定和组织模式结构的分子调控提供了一个范例。单细胞RNA测序和空间转录组学等新兴技术为解剖细胞组织、形态和分子特性的差异以及谱系分配开辟了新的途径。实验和计算工具的快速发展使研究人员能够做出许多发现并重新审视旧的假设。在这篇综述中,我们描述了单细胞RNA测序在干细胞谱系的分子轨迹和基因调控网络研究中的应用,同时强调了单细胞和空间转录组在组织谱系和胚后原肠形成过程中的分子注释中的综合实验和计算分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using Single-Cell and Spatial Transcriptomes to Understand Stem Cell Lineage Specification During Early Embryo Development.

Embryonic development and stem cell differentiation provide a paradigm to understand the molecular regulation of coordinated cell fate determination and the architecture of tissue patterning. Emerging technologies such as single-cell RNA sequencing and spatial transcriptomics are opening new avenues to dissect cell organization, the divergence of morphological and molecular properties, and lineage allocation. Rapid advances in experimental and computational tools have enabled researchers to make many discoveries and revisit old hypotheses. In this review, we describe the use of single-cell RNA sequencing in studies of molecular trajectories and gene regulation networks for stem cell lineages, while highlighting the integratedexperimental and computational analysis of single-cell and spatial transcriptomes in the molecular annotation of tissue lineages and development during postimplantation gastrulation.

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来源期刊
CiteScore
14.90
自引率
1.10%
发文量
29
期刊介绍: Since its inception in 2000, the Annual Review of Genomics and Human Genetics has been dedicated to showcasing significant developments in genomics as they pertain to human genetics and the human genome. The journal emphasizes genomic technology, genome structure and function, genetic modification, human variation and population genetics, human evolution, and various aspects of human genetic diseases, including individualized medicine.
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