miR-106B-25簇表达:人骨肉瘤和犬骨肉瘤的比较研究

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2020-03-08 eCollection Date: 2020-01-01 DOI:10.1136/vetreco-2019-000379
Leonardo Leonardi, Maria Serena Benassi, Serena Pollino, Carmen Locaputo, Laura Pazzaglia
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引用次数: 5

摘要

背景:骨肉瘤(Osteosarcoma, OS)是犬类和人类最常见的原发性恶性骨肿瘤,具有相似的遗传和临床特征。为了确定骨性骨肉瘤进展中生物学方面的异同,我们进行了一项比较研究,以创建一个模型来改善患者的预后。方法:首先,比较人和犬OS组织中miR-106b-25 (miR-106b, miR-25和miR-93-5p)簇microrna (mirna)的表达。结果:miR-25和miR-106b的表达变化与相应的正常骨无显著差异,而miR-93-5p的表达在所有骨肉瘤标本中均升高,其中犬亚群的表达水平高于人。因此,其靶蛋白p21在犬组中的免疫染色分布较弱且不均匀。鉴于miR-93-5p在所有OS标本中的高表达,我们评估了人类143B和犬DAN OS细胞对miRNA抑制的功能反应。尽管miR-93-5p抑制后p21在mRNA和蛋白水平上的表达均有所增加,但在犬OS细胞中,细胞的增殖和凋亡反应更为显著。结论:犬骨肉瘤组织和细胞系miR-93-5p的表达水平高于人骨肉瘤。此外,miR-93-5p抑制剂的引入引起了143B和DAN的细胞反应,不同的是,在犬OS细胞系中功能影响更强烈。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

miR-106B-25 Cluster expression: a comparative human and canine osteosarcoma study.

miR-106B-25 Cluster expression: a comparative human and canine osteosarcoma study.

miR-106B-25 Cluster expression: a comparative human and canine osteosarcoma study.

miR-106B-25 Cluster expression: a comparative human and canine osteosarcoma study.

Background: Osteosarcoma (OS) is the most common primary malignant bone tumour in dogs and human beings, characterised by similar genetic and clinical features. With the aim to define similarities and differences in the biological aspects involved in OS progression, a comparative study was performed to create a model to improve patient outcome.

Methods: First, the expression of microRNAs (miRNAs) belonging to the cluster miR-106b-25 (miR-106b, miR-25 and miR-93-5p) in human and canine OS tissue was compared.

Results: miR-25 and miR-106b presented a variable expression not significantly different from the corresponding normal bone, while miR-93-5p expression was increased in all OS specimens, with higher levels in the canine subset compared with human. Accordingly, its target p21 presented a weaker and less homogeneous immunostaining distribution in the canine group. Given the high expression of miR-93-5p in all OS specimens, the functional response of human 143B and canine DAN OS cells to miRNA inhibition was evaluated. Although p21 expression increased after miR-93-5p inhibition both at mRNA and protein level, a more significant cell response in terms of proliferation and apoptosis was seen in canine OS cells.

Conclusions: In conclusion, canine OS tissue and cell line presented higher expression levels of miR-93-5p than human OS. In addition, the introduction of miR-93-5p inhibitor caused a cell response in 143B and DAN that differed for the more intense functional impact in the canine OS cell line.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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