软团簇诱导解吸/电离质谱:软有多软?

IF 2.1 4区医学 Q2 Physics and Astronomy

Biointerphases Pub Date : 2020-03-12 DOI:10.1116/6.0000046

André Portz, Karolin Bomhardt, Marcus Rohnke, Pascal Schneider, Arndt Asperger, Christoph R Gebhardt, Michael Dürr

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引用次数: 8

摘要

中性簇诱导解吸/电离(DINeC)是一种超软解吸/电离方法，用于质谱(MS)分析脆弱生物分子。作为测试分子，我们用DINeC- ms测量了糖肽万古霉素，并将所得的质谱与电喷雾电离(ESI)、基质辅助激光解吸电离和飞行时间二次离子质谱进行了比较。在基于解吸的技术中，DINeC光谱的片段丰度与ESI光谱相比最低。在质谱分析中进一步证实了dinc的软解吸特性。

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Soft cluster-induced desorption/ionization mass spectrometry: How soft is soft?

Desorption/ionization induced by neutral clusters (DINeC) is used as an ultrasoft desorption/ionization method for the analysis of fragile biomolecules by means of mass spectrometry (MS). As a test molecule, the glycopeptide vancomycin was measured with DINeC-MS, and resulting mass spectra were compared to the results obtained with electrospray ionization (ESI), matrix assisted laser desorption ionization, and time-of-flight secondary ion MS. Of the desorption-based techniques, DINeC spectra show the lowest abundance of fragments comparable to ESI spectra. The soft desorption nature of DINeC was further demonstrated when applied to MS analysis of teicoplanin.

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来源期刊

Biointerphases BIOPHYSICS-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.