阴离子食用色素酒黄石通过调控组蛋白衍生肽DHVAR4的膜活性来增强其抗菌作用。

IF 7.2 2区 生物学 Q1 BIOPHYSICS
Maria Ricci, Kata Horváti, Tünde Juhász, Imola Szigyártó, György Török, Fanni Sebák, Andrea Bodor, László Homolya, Judit Henczkó, Bernadett Pályi, Tamás Mlinkó, Judith Mihály, Bilal Nizami, Zihuayuan Yang, Fengming Lin, Xiaolin Lu, Loránd Románszki, Attila Bóta, Zoltán Varga, Szilvia Bősze, Ferenc Zsila, Tamás Beke-Somfai
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引用次数: 7

摘要

本文展示了我们日常饮食中常用的一些阴离子食品添加剂,如酒黄(TZ),如何与DHVAR4(一种来源于口服宿主防御肽的抗菌肽(AMP))结合,从而显著促进对革兰氏阳性和革兰氏阴性细菌的毒性活性,但对哺乳动物细胞没有毒性活性。DHVAR4-TZ相互作用的生物物理研究表明,最初形成了大的、带正电的聚集体,但在脂质双分子层的存在下,它们更倾向于与膜表面结合。与混合抗菌化合物的协同作用相反,这是一个主要不同的机制,其中TZ直接作用于膜相关的AMP,促进其生物活性的螺旋构象。模型囊泡研究表明,与不含染料的DHVAR4相比,肽- tz复合物更容易与双分子层头基团区域的磷酸酯部分形成氢键,导致双分子层融合机制更受控制,协同严重的细胞损伤。抗菌肽被认为是对抗可怕的耐抗生素细菌感染的有前途的化合物;然而,我们对它们在体内的作用知之甚少,特别是它们如何与其他化学制剂相互作用。目前的例子说明了食物色素如何调节AMP活性,这有望激发针对消化道微生物感染的改进疗法。结果还表明,天然抗菌肽的结构和功能可以被小分子化合物操纵,这也可能为未来肽类抗菌素的设计提供新的策略概念。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anionic food color tartrazine enhances antibacterial efficacy of histatin-derived peptide DHVAR4 by fine-tuning its membrane activity.

Here it is demonstrated how some anionic food additives commonly used in our diet, such as tartrazine (TZ), bind to DHVAR4, an antimicrobial peptide (AMP) derived from oral host defense peptides, resulting in significantly fostered toxic activity against both Gram-positive and Gram-negative bacteria, but not against mammalian cells. Biophysical studies on the DHVAR4-TZ interaction indicate that initially large, positively charged aggregates are formed, but in the presence of lipid bilayers, they rather associate with the membrane surface. In contrast to synergistic effects observed for mixed antibacterial compounds, this is a principally different mechanism, where TZ directly acts on the membrane-associated AMP promoting its biologically active helical conformation. Model vesicle studies show that compared to dye-free DHVAR4, peptide-TZ complexes are more prone to form H-bonds with the phosphate ester moiety of the bilayer head-group region resulting in more controlled bilayer fusion mechanism and concerted severe cell damage. AMPs are considered as promising compounds to combat formidable antibiotic-resistant bacterial infections; however, we know very little on their in vivo actions, especially on how they interact with other chemical agents. The current example illustrates how food dyes can modulate AMP activity, which is hoped to inspire improved therapies against microbial infections in the alimentary tract. Results also imply that the structure and function of natural AMPs could be manipulated by small compounds, which may also offer a new strategic concept for the future design of peptide-based antimicrobials.

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来源期刊
Quarterly Reviews of Biophysics
Quarterly Reviews of Biophysics 生物-生物物理
CiteScore
12.90
自引率
1.60%
发文量
16
期刊介绍: Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.
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