epcam -claudin-tetraspanin调控卵巢癌进展及耐药。

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2020-12-01 DOI:10.1080/19336918.2020.1732761

Zehra Tavsan, Hülya Ayar Kayalı

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引用次数: 6

摘要

细胞粘附的改变参与了癌症的进展，但其进展和细胞粘附的机制仍然知之甚少。我们将重点放在EpCAM、claudin和tetraspanins之间的复合物上，描述了这些分子在卵巢癌中相互关联的一系列事件。通过免疫沉淀和免疫印迹评价分子间的相互作用。为了确定复合物形成对卵巢癌进展的影响，我们比较了不同类型的卵巢癌细胞系。在这项研究中，我们报道了EpCAM-claudin-4或-7-CD82复合物在卵巢癌进展和转移中的体外鉴定。此外，我们证明了棕榈酰化和细胞内或细胞外区域是复杂形成的关键。这些结果代表了细胞粘附分子动力学与卵巢癌进展之间联系的第一个直接证据。

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EpCAM-claudin-tetraspanin-modulated ovarian cancer progression and drug resistance.

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EpCAM-claudin-tetraspanin-modulated ovarian cancer progression and drug resistance.

ABSTRACT Alterations of cell adhesion are involved in cancer progression, but the mechanisms underlying the progression and cell adhesion have remained poorly understood. Focusing on the complex between EpCAM, claudins and tetraspanins, we described a sequence of events by which of the molecules associate each other in ovarian cancer. The interactions between molecules were evaluated by immunoprecipitations and then immunoblotting. To identify the effects of complex formation on the ovarian cancer progression, the different types of ovarian cancer cell lines were compared. In this study, we report the identification of the EpCAM-claudin-4 or −7-CD82 complex in the ovarian cancer progression and metastasis in vitro. Additionally, we demonstrated palmitoylation and intra- or extra-cellular regions are critically required for the complex formation. These results represent the first direct evidence for the link between the dynamism of cell adhesion molecules and ovarian cancer progression.

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.