Yuan-Kai Tu, Han-Wei Chen, Kuang-Yu Tseng, Yen-Chun Lin, Bo-Jein Kuo
{"title":"台湾甘蓝型油菜向油菜杂交F1的形态与遗传特征。","authors":"Yuan-Kai Tu, Han-Wei Chen, Kuang-Yu Tseng, Yen-Chun Lin, Bo-Jein Kuo","doi":"10.1186/s40529-019-0279-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Unintentional introgression from genetically modified (GM) oilseed rape (Brassica napus) to a relative is inevitable in the open field. A feasible and practical strategy for restricting the spread of GM offspring is to set a reasonable isolated distance between GM B. napus and the relatives. To define the isolated distance, a pollen donor/recipient pair is a prerequisite to conducting the field trial of pollen flow. However, because the cultivation of GM B. napus is prohibited in Taiwan, it is difficult to obtain relevant information. Thus, this study explored the morphological and genetic characteristics of five varieties of B. napus (donor), three varieties of B. rapa (recipient), and the 15 corresponding F<sub>1</sub> hybrids, aiming to construct phenotypic data and genetic variation data and to select the most appropriate pollen donor/recipient for future field trials of pollen flow.</p><p><strong>Results: </strong>The genome size of all F<sub>1</sub> hybrids estimated using flow cytometry showed intermediate DNA content between B. napus and B. rapa varieties. Most of the F<sub>1</sub> hybrids had intermediate plant height and blooming period, and the rosette leaves type and colors resembled those of B. napus varieties. The results of sequence-related amplified polymorphism (SRAP) showed an average of 9.52 bands per primer combination and 67.87 polymorphic bands among the F<sub>1</sub> hybrid population. Similarity and cluster analyses revealed higher similarity between F<sub>1</sub> hybrids and B. napus varieties than between F<sub>1</sub> hybrids and B. rapa varieties. Furthermore, we identified a specific 1100-bp band (LOC106302894) in F<sub>1</sub> hybrids and B. napus varieties but not in B. rapa varieties.</p><p><strong>Conclusions: </strong>The rosette leaves and the DNA marker LOC106302894 observed in F<sub>1</sub> hybrids are consistent phenotypic and genetic characteristics that can be used to identify the presence of unintentional hybridization from B. napus to B. rapa in Taiwan. Due to the prohibition of GM crop cultivation, the hybridization system of non-GM Brassica species in this study can be utilized as a mimic scheme to conduct pollen flow trials, thus facilitating the determination of the proper isolated distance.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-019-0279-5","citationCount":"9","resultStr":"{\"title\":\"Morphological and genetic characteristics of F<sub>1</sub> hybrids introgressed from Brassica napus to B. rapa in Taiwan.\",\"authors\":\"Yuan-Kai Tu, Han-Wei Chen, Kuang-Yu Tseng, Yen-Chun Lin, Bo-Jein Kuo\",\"doi\":\"10.1186/s40529-019-0279-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Unintentional introgression from genetically modified (GM) oilseed rape (Brassica napus) to a relative is inevitable in the open field. A feasible and practical strategy for restricting the spread of GM offspring is to set a reasonable isolated distance between GM B. napus and the relatives. To define the isolated distance, a pollen donor/recipient pair is a prerequisite to conducting the field trial of pollen flow. However, because the cultivation of GM B. napus is prohibited in Taiwan, it is difficult to obtain relevant information. Thus, this study explored the morphological and genetic characteristics of five varieties of B. napus (donor), three varieties of B. rapa (recipient), and the 15 corresponding F<sub>1</sub> hybrids, aiming to construct phenotypic data and genetic variation data and to select the most appropriate pollen donor/recipient for future field trials of pollen flow.</p><p><strong>Results: </strong>The genome size of all F<sub>1</sub> hybrids estimated using flow cytometry showed intermediate DNA content between B. napus and B. rapa varieties. Most of the F<sub>1</sub> hybrids had intermediate plant height and blooming period, and the rosette leaves type and colors resembled those of B. napus varieties. The results of sequence-related amplified polymorphism (SRAP) showed an average of 9.52 bands per primer combination and 67.87 polymorphic bands among the F<sub>1</sub> hybrid population. Similarity and cluster analyses revealed higher similarity between F<sub>1</sub> hybrids and B. napus varieties than between F<sub>1</sub> hybrids and B. rapa varieties. Furthermore, we identified a specific 1100-bp band (LOC106302894) in F<sub>1</sub> hybrids and B. napus varieties but not in B. rapa varieties.</p><p><strong>Conclusions: </strong>The rosette leaves and the DNA marker LOC106302894 observed in F<sub>1</sub> hybrids are consistent phenotypic and genetic characteristics that can be used to identify the presence of unintentional hybridization from B. napus to B. rapa in Taiwan. Due to the prohibition of GM crop cultivation, the hybridization system of non-GM Brassica species in this study can be utilized as a mimic scheme to conduct pollen flow trials, thus facilitating the determination of the proper isolated distance.</p>\",\"PeriodicalId\":48844,\"journal\":{\"name\":\"Botanical Studies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2020-01-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s40529-019-0279-5\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Botanical Studies\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s40529-019-0279-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Botanical Studies","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s40529-019-0279-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Morphological and genetic characteristics of F1 hybrids introgressed from Brassica napus to B. rapa in Taiwan.
Background: Unintentional introgression from genetically modified (GM) oilseed rape (Brassica napus) to a relative is inevitable in the open field. A feasible and practical strategy for restricting the spread of GM offspring is to set a reasonable isolated distance between GM B. napus and the relatives. To define the isolated distance, a pollen donor/recipient pair is a prerequisite to conducting the field trial of pollen flow. However, because the cultivation of GM B. napus is prohibited in Taiwan, it is difficult to obtain relevant information. Thus, this study explored the morphological and genetic characteristics of five varieties of B. napus (donor), three varieties of B. rapa (recipient), and the 15 corresponding F1 hybrids, aiming to construct phenotypic data and genetic variation data and to select the most appropriate pollen donor/recipient for future field trials of pollen flow.
Results: The genome size of all F1 hybrids estimated using flow cytometry showed intermediate DNA content between B. napus and B. rapa varieties. Most of the F1 hybrids had intermediate plant height and blooming period, and the rosette leaves type and colors resembled those of B. napus varieties. The results of sequence-related amplified polymorphism (SRAP) showed an average of 9.52 bands per primer combination and 67.87 polymorphic bands among the F1 hybrid population. Similarity and cluster analyses revealed higher similarity between F1 hybrids and B. napus varieties than between F1 hybrids and B. rapa varieties. Furthermore, we identified a specific 1100-bp band (LOC106302894) in F1 hybrids and B. napus varieties but not in B. rapa varieties.
Conclusions: The rosette leaves and the DNA marker LOC106302894 observed in F1 hybrids are consistent phenotypic and genetic characteristics that can be used to identify the presence of unintentional hybridization from B. napus to B. rapa in Taiwan. Due to the prohibition of GM crop cultivation, the hybridization system of non-GM Brassica species in this study can be utilized as a mimic scheme to conduct pollen flow trials, thus facilitating the determination of the proper isolated distance.
期刊介绍:
Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.