台湾甘蓝型油菜向油菜杂交F1的形态与遗传特征。

IF 4.1 3区 生物学 Q1 PLANT SCIENCES
Yuan-Kai Tu, Han-Wei Chen, Kuang-Yu Tseng, Yen-Chun Lin, Bo-Jein Kuo
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引用次数: 9

摘要

背景:转基因油菜(Brassica napus)无意中向其亲缘种渗透是在开阔田地中不可避免的。在甘蓝型甘蓝型油菜与近缘种之间设置合理的隔离距离是限制转基因后代传播的一种切实可行的策略。为了确定隔离距离,花粉供体/受体对是进行花粉流田间试验的先决条件。然而,由于台湾禁止种植转基因甘蓝型油菜,因此很难获得相关信息。因此,本研究对5个甘蓝型油菜品种(供体)、3个甘蓝型油菜品种(受体)及其15个F1杂交品种的形态和遗传特征进行了研究,旨在构建表型数据和遗传变异数据,为今后花粉流田间试验选择最合适的花粉供体/受体。结果:用流式细胞术估计的F1杂交种的基因组大小显示,DNA含量介于甘蓝型油菜和油菜品种之间。大多数F1杂交种株高和花期中等,莲座叶的类型和颜色与甘蓝型相近。序列相关扩增多态性(SRAP)分析结果显示,每个引物组合平均有9.52条多态性带,F1杂交群体平均有67.87条多态性带。相似分析和聚类分析结果表明,F1杂交种与油菜品种之间的相似性高于F1杂交种与油菜品种之间的相似性。此外,我们在F1杂交种和油菜品种中发现了一个特异的1100 bp的条带(LOC106302894),而油菜品种中没有。结论:在F1杂交品种中观察到的莲座叶和DNA标记LOC106302894具有一致的表型和遗传特征,可用于鉴定台湾地区甘蓝型油菜与油菜之间存在非故意杂交。由于禁止转基因作物种植,本研究非转基因芸苔属植物杂交系统可作为模拟方案进行花粉流试验,便于确定适宜的隔离距离。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Morphological and genetic characteristics of F<sub>1</sub> hybrids introgressed from Brassica napus to B. rapa in Taiwan.

Morphological and genetic characteristics of F<sub>1</sub> hybrids introgressed from Brassica napus to B. rapa in Taiwan.

Morphological and genetic characteristics of F<sub>1</sub> hybrids introgressed from Brassica napus to B. rapa in Taiwan.

Morphological and genetic characteristics of F1 hybrids introgressed from Brassica napus to B. rapa in Taiwan.

Background: Unintentional introgression from genetically modified (GM) oilseed rape (Brassica napus) to a relative is inevitable in the open field. A feasible and practical strategy for restricting the spread of GM offspring is to set a reasonable isolated distance between GM B. napus and the relatives. To define the isolated distance, a pollen donor/recipient pair is a prerequisite to conducting the field trial of pollen flow. However, because the cultivation of GM B. napus is prohibited in Taiwan, it is difficult to obtain relevant information. Thus, this study explored the morphological and genetic characteristics of five varieties of B. napus (donor), three varieties of B. rapa (recipient), and the 15 corresponding F1 hybrids, aiming to construct phenotypic data and genetic variation data and to select the most appropriate pollen donor/recipient for future field trials of pollen flow.

Results: The genome size of all F1 hybrids estimated using flow cytometry showed intermediate DNA content between B. napus and B. rapa varieties. Most of the F1 hybrids had intermediate plant height and blooming period, and the rosette leaves type and colors resembled those of B. napus varieties. The results of sequence-related amplified polymorphism (SRAP) showed an average of 9.52 bands per primer combination and 67.87 polymorphic bands among the F1 hybrid population. Similarity and cluster analyses revealed higher similarity between F1 hybrids and B. napus varieties than between F1 hybrids and B. rapa varieties. Furthermore, we identified a specific 1100-bp band (LOC106302894) in F1 hybrids and B. napus varieties but not in B. rapa varieties.

Conclusions: The rosette leaves and the DNA marker LOC106302894 observed in F1 hybrids are consistent phenotypic and genetic characteristics that can be used to identify the presence of unintentional hybridization from B. napus to B. rapa in Taiwan. Due to the prohibition of GM crop cultivation, the hybridization system of non-GM Brassica species in this study can be utilized as a mimic scheme to conduct pollen flow trials, thus facilitating the determination of the proper isolated distance.

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来源期刊
Botanical Studies
Botanical Studies PLANT SCIENCES-
CiteScore
4.80
自引率
2.90%
发文量
32
审稿时长
13 weeks
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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