Rashid Jahangirnejad, Mehdi Goudarzi, Heibatullah Kalantari, Hossein Najafzadeh, Mohsen Rezaei
{"title":"砷纳米颗粒在离体大鼠肝细胞中引起的亚细胞器毒性","authors":"Rashid Jahangirnejad, Mehdi Goudarzi, Heibatullah Kalantari, Hossein Najafzadeh, Mohsen Rezaei","doi":"10.15171/ijoem.2020.1614","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent.</p><p><strong>Objective: </strong>To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes.</p><p><strong>Methods: </strong>Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 μM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated.</p><p><strong>Results: </strong>By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged.</p><p><strong>Conclusion: </strong>Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.</p>","PeriodicalId":46545,"journal":{"name":"International Journal of Occupational and Environmental Medicine","volume":"11 1","pages":"41-52"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/94/ijoem-11-41.PMC7024596.pdf","citationCount":"0","resultStr":"{\"title\":\"Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes.\",\"authors\":\"Rashid Jahangirnejad, Mehdi Goudarzi, Heibatullah Kalantari, Hossein Najafzadeh, Mohsen Rezaei\",\"doi\":\"10.15171/ijoem.2020.1614\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent.</p><p><strong>Objective: </strong>To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes.</p><p><strong>Methods: </strong>Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 μM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated.</p><p><strong>Results: </strong>By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged.</p><p><strong>Conclusion: </strong>Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.</p>\",\"PeriodicalId\":46545,\"journal\":{\"name\":\"International Journal of Occupational and Environmental Medicine\",\"volume\":\"11 1\",\"pages\":\"41-52\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/94/ijoem-11-41.PMC7024596.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Occupational and Environmental Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15171/ijoem.2020.1614\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Occupational and Environmental Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15171/ijoem.2020.1614","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes.
Background: Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent.
Objective: To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes.
Methods: Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 μM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated.
Results: By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged.
Conclusion: Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
