单核增生李斯特菌纤维二糖转运组分及其对毒力基因抑制作用的研究。

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology
Thanh Nguyen Cao, Philippe Joyet, Francine Moussan Désirée Aké, Eliane Milohanic, Josef Deutscher
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引用次数: 11

摘要

背景:许多细菌通过磷酸烯醇丙酮酸:碳水化合物磷酸转移酶系统(PTS)转运纤维素二糖。在单核增生李斯特菌中,两对可溶性PTS组分(EIIACel1/EIIBCel1和EIIACel2/EIIBCel2)和渗透酶EIICCel1被认为有助于纤维二糖的摄取。有趣的是,利用几种碳水化合物,包括纤维素二糖,通过抑制毒力基因激活因子PrfA,强烈抑制毒力基因的表达。结果:类水平转录调节因子CelR激活了纤维素糖诱导的PTS操纵子celB1-celC1-celA1、celB2-celA2和编码eiic的单顺反子celC2的表达。P ~ His-HPr在His550位点的磷酸化激活了CelR,而P ~ EIIBCel1或P ~ EIIBCel2在His823位点的磷酸化抑制了它。用Ala替代His823或同时缺失celA或celB基因可导致组成型CelR调控表达。缺乏EIICCel1、CelR或两者都缺乏的突变体表现出缓慢的纤维素糖消耗。celC1或celR的缺失阻止了双糖对毒力基因的抑制,而葡萄糖和果糖则不起作用。令人惊讶的是,即使在非抑制性碳水化合物的生长过程中,两种celA基因的缺失也会导致毒力基因的抑制。celC2突变体未发现纤维素二糖相关表型。结论:两个EIIA/BCel对在eiiccel1催化的纤维二糖运输和CelR调控中作为磷酸化基供体具有相似的效率。celA双突变体的永久毒力基因抑制进一步支持PTSCel成分在PrfA调控中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies of the Listeria monocytogenes Cellobiose Transport Components and Their Impact on Virulence Gene Repression.

Background: Many bacteria transport cellobiose via a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In Listeria monocytogenes, two pairs of soluble PTS components (EIIACel1/EIIBCel1 and EIIACel2/EIIBCel2) and the permease EIICCel1 were suggested to contribute to cellobiose uptake. Interestingly, utilization of several carbohydrates, including cellobiose, strongly represses virulence gene expression by inhibiting PrfA, the virulence gene activator.

Results: The LevR-like transcription regulator CelR activates expression of the cellobiose-induced PTS operons celB1-celC1-celA1, celB2-celA2, and the EIIC-encoding monocistronic celC2. Phosphorylation by P∼His-HPr at His550 activates CelR, whereas phosphorylation by P∼EIIBCel1 or P∼EIIBCel2 at His823 inhibits it. Replacement of His823 with Ala or deletion of both celA or celB genes caused constitutive CelR regulon expression. Mutants lacking EIICCel1, CelR or both EIIACel exhibitedslow cellobiose consumption. Deletion of celC1 or celR prevented virulence gene repression by the disaccharide, but not by glucose and fructose. Surprisingly, deletion of both celA genes caused virulence gene repression even during growth on non-repressing carbohydrates. No cellobiose-related phenotype was found for the celC2 mutant.

Conclusion: The two EIIA/BCel pairs are similarly efficient as phosphoryl donors in EIICCel1-catalyzed cellobiose transport and CelR regulation. The permanent virulence gene repression in the celA double mutant further supports a role of PTSCel components in PrfA regulation.

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来源期刊
Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物
CiteScore
3.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.
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