利用新一代测序技术确定马肠道蠕虫的多样性:鉴定马的“nemabiome”。

Q3 Veterinary
Journal of Equine Science Pub Date : 2019-03-01 Epub Date: 2019-04-03 DOI:10.1294/jes.30.1
Catriona J Mitchell, Catherine M O'Sullivan, Eric Pinloche, Toby Wilkinson, Russell M Morphew, Neil R McEwan
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引用次数: 15

摘要

来自线虫卵的下一代DNA测序已被用于首次描述马的“线虫组”。在所有调查的马粪便样本中,均检测到多种圆形菌科,从7.5种(SEM 0.79)到13.3种(SEM 0.80),与NCBI数据库中的序列同源性为99%以上。这个范围是以前形态学研究中描述的物种数量的典型,每只动物使用大量的食糜。然而,目前的方法是非侵入性的;依赖于DNA分析,避免了对专业显微镜鉴定的需要;并且可以用小样本进行,与现有方法相比具有显着优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine 'nemabiome'.

Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine 'nemabiome'.

Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine 'nemabiome'. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.

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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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