楼梯测试中的长期精细运动能力与尾壳核中darpp阳性神经元的绝对数量相关,而与密度无关。

IF 2.1
Anatomical record (Hoboken, N.J. : 2007) Pub Date : 2019-11-01 Epub Date: 2019-06-28 DOI:10.1002/ar.24196
Benjamin E Aghoghovwia, Liping Goddard, Dorothy E Oorschot
{"title":"楼梯测试中的长期精细运动能力与尾壳核中darpp阳性神经元的绝对数量相关,而与密度无关。","authors":"Benjamin E Aghoghovwia,&nbsp;Liping Goddard,&nbsp;Dorothy E Oorschot","doi":"10.1002/ar.24196","DOIUrl":null,"url":null,"abstract":"<p><p>Measurement of long-term functional and anatomical outcomes in the same animal is considered a powerful strategy for correlating structure with function. In a neonatal animal model of hypoxic-ischemic brain injury that is relevant to cerebral palsy, long-term functional deficits on the staircase test and long-term anatomical deficits in the absolute number of medium-spiny projection neurons in the caudate-putamen were reported in different animals due to logistical constraints. Here, we investigated if these functional and anatomical measures were correlated when measured in the same animals. The medium-spiny projection neurons were investigated because (1) they comprise the vast majority (>97%) of all neurons in the caudate-putamen and (2) motor deficits observed during staircase testing are likely to involve these striatal medium-spiny projection neurons through their connections. We found that long-term skilled forepaw capability on the staircase test was correlated with the absolute number of DARPP-32-positive medium-spiny projection neurons in the caudate-putamen. Specifically, deficits in skilled forepaw ability for the number of sugar pellets eaten and retrieved, and for the maximum staircase level reached, were significantly correlated with a lower absolute neuronal number. We also found that skilled forepaw ability on the staircase test was not correlated with the neuronal density (i.e., number per unit volume) of DARPP-32-positive medium-spiny projection neurons. Since neuronal density is an indirect measure of neuronal survival that is used in the literature, and absolute neuronal number is a direct measure, the results also highlight the scientific value of measuring absolute neuronal number. Anat Rec, 302:2040-2048, 2019. © 2019 American Association for Anatomy.</p>","PeriodicalId":520555,"journal":{"name":"Anatomical record (Hoboken, N.J. : 2007)","volume":" ","pages":"2040-2048"},"PeriodicalIF":2.1000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.24196","citationCount":"2","resultStr":"{\"title\":\"Long-Term Fine Motor Capability on the Staircase Test Correlates with the Absolute Number, but Not the Density, of DARPP-Positive Neurons in the Caudate-Putamen.\",\"authors\":\"Benjamin E Aghoghovwia,&nbsp;Liping Goddard,&nbsp;Dorothy E Oorschot\",\"doi\":\"10.1002/ar.24196\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Measurement of long-term functional and anatomical outcomes in the same animal is considered a powerful strategy for correlating structure with function. In a neonatal animal model of hypoxic-ischemic brain injury that is relevant to cerebral palsy, long-term functional deficits on the staircase test and long-term anatomical deficits in the absolute number of medium-spiny projection neurons in the caudate-putamen were reported in different animals due to logistical constraints. Here, we investigated if these functional and anatomical measures were correlated when measured in the same animals. The medium-spiny projection neurons were investigated because (1) they comprise the vast majority (>97%) of all neurons in the caudate-putamen and (2) motor deficits observed during staircase testing are likely to involve these striatal medium-spiny projection neurons through their connections. We found that long-term skilled forepaw capability on the staircase test was correlated with the absolute number of DARPP-32-positive medium-spiny projection neurons in the caudate-putamen. Specifically, deficits in skilled forepaw ability for the number of sugar pellets eaten and retrieved, and for the maximum staircase level reached, were significantly correlated with a lower absolute neuronal number. We also found that skilled forepaw ability on the staircase test was not correlated with the neuronal density (i.e., number per unit volume) of DARPP-32-positive medium-spiny projection neurons. Since neuronal density is an indirect measure of neuronal survival that is used in the literature, and absolute neuronal number is a direct measure, the results also highlight the scientific value of measuring absolute neuronal number. Anat Rec, 302:2040-2048, 2019. © 2019 American Association for Anatomy.</p>\",\"PeriodicalId\":520555,\"journal\":{\"name\":\"Anatomical record (Hoboken, N.J. : 2007)\",\"volume\":\" \",\"pages\":\"2040-2048\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2019-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/ar.24196\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anatomical record (Hoboken, N.J. : 2007)\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/ar.24196\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2019/6/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomical record (Hoboken, N.J. : 2007)","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/ar.24196","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/6/28 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

摘要

测量同一动物的长期功能和解剖结果被认为是将结构与功能联系起来的有力策略。在一个与脑瘫相关的新生儿缺氧缺血性脑损伤动物模型中,由于后勤限制,在不同的动物中,报告了阶梯试验的长期功能缺陷和尾壳核中棘投射神经元绝对数量的长期解剖缺陷。在这里,我们研究了在同一动物中测量这些功能和解剖测量是否相关。研究中棘突起神经元的原因是:(1)它们占尾状壳核神经元的绝大多数(>97%),(2)在阶梯测试中观察到的运动缺陷可能与纹状体中棘突起神经元的连接有关。我们发现,在楼梯测试中,长期熟练的前爪能力与尾壳核中darpp -32阳性的中棘投射神经元的绝对数量相关。具体来说,在熟练的前爪能力中,进食和捡取糖球的数量,以及达到的最大楼梯水平的缺陷,与绝对神经元数量的减少显著相关。我们还发现,在楼梯测试中熟练的前爪能力与神经元密度(即每单位体积的数量)darpp -32阳性中棘投射神经元无关。由于文献中使用的神经元密度是神经元存活的间接度量,而绝对神经元数是直接度量,因此该结果也突出了测量绝对神经元数的科学价值。生物医学工程学报,2016,32(2):407 - 408。©2019美国解剖学协会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Long-Term Fine Motor Capability on the Staircase Test Correlates with the Absolute Number, but Not the Density, of DARPP-Positive Neurons in the Caudate-Putamen.

Measurement of long-term functional and anatomical outcomes in the same animal is considered a powerful strategy for correlating structure with function. In a neonatal animal model of hypoxic-ischemic brain injury that is relevant to cerebral palsy, long-term functional deficits on the staircase test and long-term anatomical deficits in the absolute number of medium-spiny projection neurons in the caudate-putamen were reported in different animals due to logistical constraints. Here, we investigated if these functional and anatomical measures were correlated when measured in the same animals. The medium-spiny projection neurons were investigated because (1) they comprise the vast majority (>97%) of all neurons in the caudate-putamen and (2) motor deficits observed during staircase testing are likely to involve these striatal medium-spiny projection neurons through their connections. We found that long-term skilled forepaw capability on the staircase test was correlated with the absolute number of DARPP-32-positive medium-spiny projection neurons in the caudate-putamen. Specifically, deficits in skilled forepaw ability for the number of sugar pellets eaten and retrieved, and for the maximum staircase level reached, were significantly correlated with a lower absolute neuronal number. We also found that skilled forepaw ability on the staircase test was not correlated with the neuronal density (i.e., number per unit volume) of DARPP-32-positive medium-spiny projection neurons. Since neuronal density is an indirect measure of neuronal survival that is used in the literature, and absolute neuronal number is a direct measure, the results also highlight the scientific value of measuring absolute neuronal number. Anat Rec, 302:2040-2048, 2019. © 2019 American Association for Anatomy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信