J M Lorch, J M Palmer, K J Vanderwolf, K Z Schmidt, M L Verant, T J Weller, D S Blehert
{"title":"从蝙蝠中分离的一种耐寒酵母。","authors":"J M Lorch, J M Palmer, K J Vanderwolf, K Z Schmidt, M L Verant, T J Weller, D S Blehert","doi":"10.3767/persoonia.2018.41.04","DOIUrl":null,"url":null,"abstract":"<p><p><i>Malassezia</i> is a genus of medically-important, lipid-dependent yeasts that live on the skin of warm-blooded animals. The 17 described species have been documented primarily on humans and domestic animals, but few studies have examined <i>Malassezia</i> species associated with more diverse host groups such as wildlife. While investigating the skin mycobiota of healthy bats, we isolated a <i>Malassezia</i> sp. that exhibited only up to 92% identity with other known species in the genus for the portion of the DNA sequence of the internal transcribed spacer region that could be confidently aligned. The <i>Malassezia</i> sp. was cultured from the skin of nine species of bats in the subfamily <i>Myotinae</i>; isolates originated from bats sampled in both the eastern and western United States. Physiological features and molecular characterisation at seven additional loci (D1/D2 region of 26S rDNA, 18S rDNA, chitin synthase, second largest subunit of RNA polymerase II, β-tubulin, translation elongation factor EF-1α, and minichromosome maintenance complex component 7) indicated that all of the bat <i>Malassezia</i> isolates likely represented a single species distinct from other named taxa. Of particular note was the ability of the <i>Malassezia</i> sp. to grow over a broad range of temperatures (7-40 °C), with optimal growth occurring at 24 °C. These thermal growth ranges, unique among the described <i>Malassezia</i>, may be an adaptation by the fungus to survive on bats during both the host's hibernation and active seasons. The combination of genetic and physiological differences provided compelling evidence that this lipid-dependent yeast represents a novel species described herein as <i>Malassezia vespertilionis</i> sp. nov. Whole genome sequencing placed the new species as a basal member of the clade containing the species <i>M. furfur</i>, <i>M. japonica</i>, <i>M. obtusa</i>, and <i>M. yamatoensis</i>. The genetic and physiological uniqueness of <i>Malassezia vespertilionis</i> among its closest relatives may make it important in future research to better understand the evolution, life history, and pathogenicity of the <i>Malassezia</i> yeasts.</p>","PeriodicalId":20014,"journal":{"name":"Persoonia","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3767/persoonia.2018.41.04","citationCount":"68","resultStr":"{\"title\":\"<i>Malassezia vespertilionis</i> sp. nov.: a new cold-tolerant species of yeast isolated from bats.\",\"authors\":\"J M Lorch, J M Palmer, K J Vanderwolf, K Z Schmidt, M L Verant, T J Weller, D S Blehert\",\"doi\":\"10.3767/persoonia.2018.41.04\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Malassezia</i> is a genus of medically-important, lipid-dependent yeasts that live on the skin of warm-blooded animals. The 17 described species have been documented primarily on humans and domestic animals, but few studies have examined <i>Malassezia</i> species associated with more diverse host groups such as wildlife. While investigating the skin mycobiota of healthy bats, we isolated a <i>Malassezia</i> sp. that exhibited only up to 92% identity with other known species in the genus for the portion of the DNA sequence of the internal transcribed spacer region that could be confidently aligned. The <i>Malassezia</i> sp. was cultured from the skin of nine species of bats in the subfamily <i>Myotinae</i>; isolates originated from bats sampled in both the eastern and western United States. Physiological features and molecular characterisation at seven additional loci (D1/D2 region of 26S rDNA, 18S rDNA, chitin synthase, second largest subunit of RNA polymerase II, β-tubulin, translation elongation factor EF-1α, and minichromosome maintenance complex component 7) indicated that all of the bat <i>Malassezia</i> isolates likely represented a single species distinct from other named taxa. Of particular note was the ability of the <i>Malassezia</i> sp. to grow over a broad range of temperatures (7-40 °C), with optimal growth occurring at 24 °C. These thermal growth ranges, unique among the described <i>Malassezia</i>, may be an adaptation by the fungus to survive on bats during both the host's hibernation and active seasons. The combination of genetic and physiological differences provided compelling evidence that this lipid-dependent yeast represents a novel species described herein as <i>Malassezia vespertilionis</i> sp. nov. 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引用次数: 68
摘要
马拉色菌是一种在医学上很重要的、依赖于脂质的酵母菌属,生活在温血动物的皮肤上。所描述的17个物种主要记录在人类和家畜身上,但很少有研究调查与野生动物等更多样化的寄主群体有关的马拉色菌物种。在调查健康蝙蝠的皮肤真菌群时,我们分离出一种马拉色菌属,由于其内部转录间隔区DNA序列的一部分可以自信地排列,它与该属其他已知物种的一致性仅为92%。从蝙蝠亚科9种蝙蝠皮肤中培养马拉色菌;分离株起源于美国东部和西部取样的蝙蝠。另外7个位点(26S rDNA的D1/D2区、18S rDNA、几丁质合成酶、RNA聚合酶II的第二大亚基、β-微管蛋白、翻译延伸因子EF-1α和小染色体维持复合体组分7)的生理特征和分子特征表明,所有分离的马拉色菌可能代表一个不同于其他已命名分类群的单一物种。特别值得注意的是马拉色菌的生长温度范围很广(7-40°C),最佳生长温度为24°C。这些热生长范围在所描述的马拉色菌中是独特的,可能是真菌在蝙蝠的冬眠和活动季节生存的一种适应。遗传和生理差异的结合提供了令人信服的证据,证明这种脂质依赖性酵母代表了一种新物种,本文将其描述为Malassezia vespertilionis sp. 11 .全基因组测序将该新物种定位为包含M. furfur, M. japonica, M. obtusa和M. yamatoensis的分支的基础成员。马拉色菌在其近亲中的遗传和生理独特性,可能对今后研究马拉色菌的进化、生活史和致病性具有重要意义。
Malassezia vespertilionis sp. nov.: a new cold-tolerant species of yeast isolated from bats.
Malassezia is a genus of medically-important, lipid-dependent yeasts that live on the skin of warm-blooded animals. The 17 described species have been documented primarily on humans and domestic animals, but few studies have examined Malassezia species associated with more diverse host groups such as wildlife. While investigating the skin mycobiota of healthy bats, we isolated a Malassezia sp. that exhibited only up to 92% identity with other known species in the genus for the portion of the DNA sequence of the internal transcribed spacer region that could be confidently aligned. The Malassezia sp. was cultured from the skin of nine species of bats in the subfamily Myotinae; isolates originated from bats sampled in both the eastern and western United States. Physiological features and molecular characterisation at seven additional loci (D1/D2 region of 26S rDNA, 18S rDNA, chitin synthase, second largest subunit of RNA polymerase II, β-tubulin, translation elongation factor EF-1α, and minichromosome maintenance complex component 7) indicated that all of the bat Malassezia isolates likely represented a single species distinct from other named taxa. Of particular note was the ability of the Malassezia sp. to grow over a broad range of temperatures (7-40 °C), with optimal growth occurring at 24 °C. These thermal growth ranges, unique among the described Malassezia, may be an adaptation by the fungus to survive on bats during both the host's hibernation and active seasons. The combination of genetic and physiological differences provided compelling evidence that this lipid-dependent yeast represents a novel species described herein as Malassezia vespertilionis sp. nov. Whole genome sequencing placed the new species as a basal member of the clade containing the species M. furfur, M. japonica, M. obtusa, and M. yamatoensis. The genetic and physiological uniqueness of Malassezia vespertilionis among its closest relatives may make it important in future research to better understand the evolution, life history, and pathogenicity of the Malassezia yeasts.
期刊介绍:
Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.