高砷地质背景农业系统中玉米根际微生物群落对外源抗生素的抗性谱

IF 4.3 3区 环境科学与生态学 Q1 CHEMISTRY, ANALYTICAL
Moxin Xu, Jinping Jiang, Ying Feng, Xiaofeng Li, Lili Ye and Yongshan Chen
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引用次数: 0

摘要

金属(类)可以通过共选择效应增加抗生素耐药性在环境系统中的传播和富集。将抗生素引入环境对微生物群落对金属(类)化合物的长期耐药性的影响在很大程度上是未知的。在这里,将含有土霉素(OTC)或磺胺嘧啶(SD)的四种浓度(0、1、10和100 mg kg?1)的粪肥加入到具有高砷地质背景的地区的玉米种植系统中。结果表明,外源抗生素的引入对玉米根际土壤细菌多样性有显著影响,Chao1和Shannon指数与对照相比发生了变化。除了放线菌外,暴露于土霉素并没有显著改变大多数细菌门类的患病率。然而,磺胺嘧啶抗生素暴露导致患病率随着暴露浓度的增加而下降,但双胞菌除外。在5个最常见的属中观察到相同的反应模式,如Gemmatimonas、Fulvimonas、Luteimonas、Massilia和Streptomyces。结果发现,tetC、tetG和sul2抗生素耐药基因(ARGs)的丰度随着抗生素暴露浓度的增加而显著增加,并且这些基因与整合子(int1)之间存在很强的联系。与砷转化相关的微生物功能基因(aioA和arsM)丰度随着土霉素暴露浓度的增加而增加,而随着磺胺嘧啶暴露浓度的增加而减少。变形菌门、放线菌门、酸杆菌门、绿菌门、厚壁菌门、拟杆菌门、单胞菌门、蓝藻门和植菌门是抗生素引入的重要指标,可能是高砷地质背景土壤中抗生素耐药性发展的重要指标。plananctomycetacia(来自plananctomycetes)与sul2和int1基因呈显著负相关,这可能在外源抗生素耐药谱的形成中起作用。本研究将扩大我们对高地质背景地区微生物对抗生素污染耐药性的认识,并揭示复合污染的潜在生态效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Resistance profiles of microbial communities in maize rhizospheres to the introduction of exogenous antibiotics to agricultural systems with a high arsenic geological background†

Resistance profiles of microbial communities in maize rhizospheres to the introduction of exogenous antibiotics to agricultural systems with a high arsenic geological background†

Metal(loid)s can increase the spread and enrichment of antibiotic resistance in the environmental system by means of a co-selection effect. The effects of introducing antibiotics into the environment on the long-term resistance of microbial communities to metal(loid)s are largely unknown. Here, manure-fertilizers that contained either oxytetracycline (OTC) or sulfadiazine (SD) at four concentrations (0, 1, 10, and 100 mg kg?1) were incorporated into a maize cropping system in an area with a high arsenic geological background. The results showed that the introduction of exogenous antibiotics had a notable effect on the bacterial diversity of the maize rhizosphere soil, as evidenced by alterations in Chao1 and Shannon index values when compared to those of the control. Oxytetracycline exposure did not significantly alter the prevalence of most bacterial phyla, with the exception of Actinobacteria. However, sulfadiazine antibiotic exposure caused a decrease in prevalence as exposure concentrations increased, with the exception of Gemmatimonadetes. The same reaction pattern was observed in the five most prevalent genera, such as Gemmatimonas, Fulvimonas, Luteimonas, Massilia, and Streptomyces. It was observed that the abundance of tetC, tetG, and sul2 antibiotic resistance genes (ARGs) significantly increased in correlation with the concentration of antibiotic exposure, and a strong link was found between these genes and integrons (intl1). The abundance of microbial functional genes related to arsenic transformation (aioA and arsM) increased when there was an increase in oxytetracycline exposure concentrations, whereas a decrease in abundance was observed with increasing sulfadiazine exposure concentrations. Proteobacteria, Actinobacteriota, Acidobacteriota, Chloroflexi, Firmicutes, Bacteroidota, Gemmatimonadota, Cyanobacteria and Planctomycetes were found to be important indicators of the introduction of antibiotics, and may be essential in the development of antibiotic resistance in soils with high arsenic geological background. Planctomycetacia (from Planctomycetes) was significantly negatively correlated with sul2 and intl1 genes, which might play a role in the development of resistance profiles to exogenous antibiotics. This study will expand our understanding of microbial resistance to antibiotic contamination in areas with a high geological background, as well as reveal the hidden ecological effects of combined contamination.

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来源期刊
Environmental Science: Processes & Impacts
Environmental Science: Processes & Impacts CHEMISTRY, ANALYTICAL-ENVIRONMENTAL SCIENCES
CiteScore
9.50
自引率
3.60%
发文量
202
审稿时长
1 months
期刊介绍: Environmental Science: Processes & Impacts publishes high quality papers in all areas of the environmental chemical sciences, including chemistry of the air, water, soil and sediment. We welcome studies on the environmental fate and effects of anthropogenic and naturally occurring contaminants, both chemical and microbiological, as well as related natural element cycling processes.
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