醛糖还原酶(ALR2)调控基因多态性是印尼巴厘岛2型糖尿病患者糖尿病视网膜病变的危险因素

IF 0.4 Q4 OPHTHALMOLOGY
Open Ophthalmology Journal Pub Date : 2018-10-18 eCollection Date: 2018-01-01 DOI:10.2174/1874364101812010281
Desak Made Wihandani, Ketut Suastika, I Nyoman Agus Bagiada, Safarina G Malik
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引用次数: 9

摘要

目的:研究醛糖还原酶调控基因多态性是否为巴厘岛2型糖尿病(T2DM)患者糖尿病视网膜病变(DR)的危险因素。方法:这是一项病例对照研究,包括35例伴有DR的T2DM患者与35例非DR患者作为对照。采用PCR分析和dna测序检测醛糖还原酶(ALR2)基因调控区C(-106)T和C(-12)G多态性。基因型和等位基因分布采用卡方检验,其他数据采用独立t检验和Mann-Whitney U检验。结果:除了收缩压、空腹血糖和餐后2小时血糖外,两组所有受试者的基线特征均相同。本研究在ALR2基因的调控区发现了C(-104)T和C(-9)G两个多态性。结果表明,C(-104)T多态性是发生DR的危险因素(OR=36;95% ci = 4.43-292.85;p=0.001),但C(-9)G多态性不存在(OR=1.28;95%可信区间= 0.48 - -3.38;p = 0.621)。其他研究结果显示CC/CC单倍型是DR的保护因子(OR=0.198;p=0.002),而CT/CC和CT/CG单倍型是DR的危险因素,OR=15.58;p=0.002, OR=2.29;分别p = 0.005。结论:醛糖还原酶(ALR2)基因调控区C(-104)T多态性是印尼巴厘岛地区T2DM患者发生DR的危险因素。然而,小样本量、收缩压、空腹血糖和餐后2小时血糖可能会影响我们的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Polymorphisms of Aldose Reductase (ALR2) Regulatory Gene are Risk Factors for Diabetic Retinopathy in Type-2 Diabetes Mellitus Patients in Bali, Indonesia.

Polymorphisms of Aldose Reductase (ALR2) Regulatory Gene are Risk Factors for Diabetic Retinopathy in Type-2 Diabetes Mellitus Patients in Bali, Indonesia.

Aim: The study aimed to elucidate whether the polymorphisms of the aldose reductase regulatory gene were risk factors for Diabetic Retinopathy (DR) in type-2 diabetes mellitus (T2DM) patients in Bali.

Methods: This is a case-control study including 35 cases of T2DM patients with DR paired with 35 cases with non-DR as controls. PCR analysis and DNA-sequencing were carried out to detect the C(-106)T and C(-12)G polymorphisms at the regulatory region of Aldose Reductase (ALR2) gene. Genotype and allele distributions were analyzed by Chi-squared test and independent t-and Mann-Whitney U tests were used to analyze other data.

Results: Among all subjects in both groups, the baseline characteristics were homogenous except for systolic blood pressure, fasting blood glucose and 2-hours post-prandial blood glucose. This study found two polymorphisms, C(-104)T and C(-9)G, in the regulatory region of ALR2 gene. The result showed that the C(-104)T polymorphism was a risk factor for DR (OR=36; 95% CI = 4.43-292.85; p=0.001), but not the C(-9)G polymorphism (OR=1.28; 95% CI=0.48-3.38; p=0.621). Other findings in the study revealed that CC/CC haplotype is a protective factor for DR (OR=0.198; p=0.002), whereas CT/CC and CT/CG haplotypes as risk factors for DR with OR=15.58; p=0.002 and OR=2.29; p=0.005 respectively.

Conclusion: It can be concluded that C(-104)T polymorphism in the regulatory region of Aldose Reductase (ALR2) gene was the risk factor for DR among T2DM patients in Bali, Indonesia. However, small sample size, systolic blood pressure, fasting blood glucose and 2-hours post-prandial blood glucose could affect our finding.

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来源期刊
CiteScore
0.50
自引率
0.00%
发文量
24
期刊介绍: The Open Ophthalmology Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, letters and guest edited single topic issues in all important areas of experimental and clinical research in ophthalmology, including use of ophthalmological therapies, devices and surgical techniques. The Open Ophthalmology Journal, a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and making them freely available to researchers worldwide.
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