缺镁可增加脂多糖诱导的炎症,增强人脐静脉内皮细胞单核细胞粘附。

IF 1.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lujain A Almousa, Andrew M Salter, Simon C Langley-Evans
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引用次数: 10

摘要

考虑到镁在内皮细胞中可能具有抗炎作用,本研究的目的是研究镁对人脐静脉内皮细胞(HUVEC)活力、基因表达和细菌内毒素(LPS)引起的促炎反应的影响。在三种不同浓度的硫酸镁(0.1 mM;control-1 mM;5毫米)72小时。在低镁培养中,将细胞暴露于LPS会降低细胞活力,但高镁可以保护huvec免受LPS诱导的细胞死亡。低镁条件下lps处理的HUVECs促炎因子mRNA表达上调,IL-2、IL-3、IL-8、IL-15、MCP-1等细胞因子蛋白表达上调。这与单核细胞对细胞的更大粘附有关。相反,高镁降低了炎症因子和细胞因子的表达。研究发现,低镁浓度下LPS对许多促炎因子表达的激活作用加剧,而高镁浓度下LPS对炎症反应有部分抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Magnesium deficiency heightens lipopolysaccharide-induced inflammation and enhances monocyte adhesion in human umbilical vein endothelial cells.

Given a possible anti-inflammatory role of magnesium in endothelial cells, the aim of this study was to investigate the effects of magnesium on human umbilical vein endothelial cell (HUVEC) viability, gene expression, and the pro-inflammatory response caused by a bacterial endotoxin (LPS). HUVECs were cultured at three different concentrations of magnesium sulphate (0.1 mM; control-1 mM; 5 mM) for 72 hours. Exposing the cells to LPS reduced cell viability in culture with low magnesium, but high magnesium protected the HUVECs from LPS-induced cell death. LPS-treated HUVECs cultured in low magnesium showed up-regulation of mRNA expression for pro-inflammatory factors and the expression of cytokine proteins, including IL-2, IL-3, IL-8, IL-15 and MCP-1. This was associated with greater adhesion of monocytes to the cells. In contrast, high magnesium decreased the expression of inflammatory factors and cytokines. The study found that LPS activation of the expression of many pro-inflammatory factors is exacerbated in the presence of low magnesium concentration whilst a high magnesium concentration partly inhibited the inflammatory response to LPS.

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来源期刊
Magnesium research
Magnesium research 医学-内分泌学与代谢
CiteScore
3.50
自引率
9.40%
发文量
6
审稿时长
>12 weeks
期刊介绍: Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.
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