氯沙坦治疗后自发性高血压大鼠左心室Bax、Bcl-2、CCR-2、MCP-1、TGF-β1基因的变化

Korean Journal of Pediatrics Pub Date : 2019-03-01 Epub Date: 2018-10-24 DOI:10.3345/kjp.2018.06856
Hyeryon Lee, Kwan Chang Kim, Young Mi Hong
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引用次数: 2

摘要

目的:在自发性高血压大鼠(SHRs)肥大的左心室中发现细胞凋亡增加。尽管现有证据表明,心肌细胞凋亡可通过各种损伤(包括压力过载)诱导,但心肌细胞凋亡似乎是由成人SHRs中过量的局部血管紧张素产生引起的。通过研究氯沙坦对SHR模型Bcl相关X (Bax)、Bcl-2、趋化因子受体(CCR)-2、单核细胞趋化蛋白(MCP)-1、转化生长因子(TGF)-β1、磷酸化细胞外信号调节激酶(PERK)、连接蛋白43和钾化因子mRNA表达的影响,探讨氯沙坦对SHR模型的影响。方法:12周龄雄性大鼠分为:对照组(C)、高血压组(H)、氯沙坦组(L);氯沙坦治疗SHRs (10 mg/kg/天)5周。Western blot和逆转录聚合酶链反应检测。结果:第3周和第5周,H组Bax、CCR-2、MCP-1、TGF-β1、PERK、connexin 43蛋白和kallikrein mRNA的表达明显高于C组。氯沙坦治疗第5周后,Bax、CCR-2、MCP-1、TGF-β1、connexin 43蛋白和kallikrein mRNA的表达显著降低。氯沙坦治疗第3周和第5周后,PERK蛋白表达明显降低。第3、5周时,H组Bcl-2蛋白表达较C组明显降低。结论:氯沙坦治疗可降低SHRs中Bax、CCR-2、MCP-1、TGF-β1、PERK、connexin 43蛋白和kallikrein mRNA的表达,减少炎症和细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Changes of Bax, Bcl-2, CCR-2, MCP-1, and TGF-β1 genes in the left ventricle of spontaneously hypertensive rat after losartan treatment.

Changes of Bax, Bcl-2, CCR-2, MCP-1, and TGF-β1 genes in the left ventricle of spontaneously hypertensive rat after losartan treatment.

Changes of Bax, Bcl-2, CCR-2, MCP-1, and TGF-β1 genes in the left ventricle of spontaneously hypertensive rat after losartan treatment.

Changes of Bax, Bcl-2, CCR-2, MCP-1, and TGF-β1 genes in the left ventricle of spontaneously hypertensive rat after losartan treatment.

Purpose: Increased apoptosis was recently found in the hypertrophied left ventricle of spontaneously hypertensive rats (SHRs). Although the available evidence suggests that apoptosis can be induced in cardiac cells by various insults including pressure overload, cardiac apoptosis appears to result from an exaggerated local production of angiotensin in adult SHRs. Altered expressions of Bcl associated X (Bax), Bcl-2, chemokine receptor (CCR)-2, monocyte chemoattractant protein (MCP)-1, transforming growth factor (TGF)-β1, phosphorylated extracellular signal-regulated kinases (PERK), and connexin 43 proteins, and kallikrein mRNA were investigated to explore the effects of losartan on the SHR model.

Methods: Twelve-week-old male rats were grouped as follows: control (C), SHR (hypertension: H), and losartan (L; SHRs were treated with losartan [10 mg/kg/day] for 5 weeks). Western blot and reverse transcription polymerase chain reaction assays were performed.

Results: Expression of Bax, CCR-2, MCP-1, TGF-β1, PERK, and connexin 43 proteins, and kallikrein mRNA was significantly increased in the H group compared to that in the C group at weeks 3 and 5. Expression of Bax, CCR-2, MCP-1, TGF-β1, and connexin 43 proteins and kallikrein mRNA was significantly decreased after losartan treatment at week 5. PERK protein expression was significantly decreased after losartan treatment at weeks 3 and 5. Bcl-2 protein expression was significantly decreased in the H group compared to that in the C group at weeks 3 and 5.

Conclusion: Losartan treatment reduced expression of Bax, CCR-2, MCP-1, TGF-β1, PERK, and connexin 43 proteins, and kallikrein mRNA in SHRs, along with decreased inflammation and apoptosis.

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审稿时长
12 weeks
期刊介绍: Korean J Pediatr covers clinical and research works relevant to all aspects of child healthcare. The journal aims to serve pediatricians through the prompt publication of significant advances in any field of pediatrics and to rapidly disseminate recently updated knowledge to the public. Additionally, it will initiate dynamic, international, academic discussions concerning the major topics related to pediatrics. Manuscripts are categorized as review articles, original articles, and case reports. Areas of specific interest include: Growth and development, Neonatology, Pediatric neurology, Pediatric nephrology, Pediatric endocrinology, Pediatric cardiology, Pediatric allergy, Pediatric pulmonology, Pediatric infectious diseases, Pediatric immunology, Pediatric hemato-oncology, Pediatric gastroenterology, Nutrition, Human genetics, Metabolic diseases, Adolescence medicine, General pediatrics.
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