蛋白激酶C抑制剂选择性调节人结肠癌细胞粘附分子动力学和细胞死亡。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2018-10-11 DOI:10.1080/19336918.2018.1530933
Muzaffer Dükel, Zehra Tavsan, Duygu Erdogan, Deniz Erkan Gök, Hulya Ayar Kayali
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引用次数: 8

摘要

在结肠癌的发展过程中,蛋白激酶Cs (PKCs)参与了许多控制细胞机制的基因的调控。在这里,我们研究了细胞粘附分子和PKCs在结直肠癌进展中的变化。我们发现PKCs影响EpCAM、claudins、tetraspanins的表达。低浓度PKC抑制剂导致细胞活力下降。此外,免疫印迹和qRT-PCR分析显示,PKC抑制可抑制结肠癌细胞凋亡,诱导自噬。此外,我们观察到细胞内活性氧(ROS)、脂质过氧化和蛋白羰基水平下降,证实了ROS诱导的细胞凋亡。综上所述,我们的研究结果表明PKC信号不仅调节细胞粘附动力学,还调节细胞死亡相关机制。PKC:蛋白激酶C;EpCAM:上皮细胞粘附分子;胎牛血清;MTT: 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑);CAM:细胞粘附分子;ROS:活性氧。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells.

Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells.

Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells.

Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells.

During development of colon cancer, Protein Kinase Cs (PKCs) are involved in regulation of many genes controlling several cellular mechanisms. Here, we examined the changes in cell adhesion molecules and PKCs for colorectal cancer progression. We identified that PKCs affected expression of EpCAM, claudins, tetraspanins. Treatment with low concentrations of PKC inhibitors resulted in decreased cell viability. In addition, immunoblotting and qRT-PCR analysis showed that apoptosis was inhibited while autophagy was induced by PKC inhibition in colon cancer cells. Furthermore, we observed decreased levels of intracellular Reactive Oxygen Species (ROS), lipid peroxidation and protein carbonyl, confirming the ROS-induced apoptosis. Taken together, our results reveal that PKC signalling modulates not only cell adhesion dynamics but also cell death-related mechanisms. Abbreviations: PKC: Protein Kinase C; EpCAM: Epithelial cell adhesion molecule; FBS: fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); CAM: cell adhesion molecule; ROS: reactive oxygen species.

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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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