[THE RHYTHMIC ORGANIZATION OF THE ANNUAL DYNAMICS OF FUNCTIONAL INDICATORS OF RAT LIVER UNDER TOXIC LOAD].

Purpose: The purpose of this study was to investigate the annual dynamics of the functional state of the liver of rats with experimental hepatitis on early period of intoxication.

Materials and methods: This research was conducted for three years on 172 adult male rats Wistar. The toxic hepatitis was caused by subcutaneous injection three times a 50% oil solution of carbon tetrachloride. The studies were conducted in the winter (January), summer (June-July), spring (April) and autumn (October) on the 4th day after the last administration of the toxin. Activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the level of total lipids (TL), total bilirubin (TB), malondialdehyde (MDA) and thymol (T) using standard sets of Biotest «Lachema» were determined in the blood serum. Statistic processing of primary chronograms of investigated parameters was performed with the program “сosinor analysis.”

Results: It was found that in the control animals was dominated by annual and circannual rhythms of the liver functions and in the spectra of the studied parameters were observed several subdominant harmonic. The maxima of liver function detected to the different time intervals on an annual scale, that is, they were not coordinated with each other. On day 4 experimental hepatitis was increased the average annual of study functions (with the exception of ТL) and in their rhythmic organization was observed the inner synchronization by period and acrophase. In the spectra of the rhythms of the studied parameters mainly was determined by only the annual harmonic with acrophase in the autumn months. It can together indicate the development of a powerful pathological process in the liver.