为移植的胰岛重建一个更好的家园。

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-09-25 DOI:10.1080/15476278.2018.1517509
Daniel M Tremmel, Jon S Odorico
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引用次数: 18

摘要

糖尿病可以用β细胞替代疗法治疗,将患者的尸体胰岛移植以恢复血糖控制。尽管这是一种有效的治疗方法,但从胰腺中分离胰岛的过程需要胶原酶消化,这会破坏胰岛细胞外基质(ECM)并激活anoiki介导的细胞凋亡。为了提高胰岛在培养和移植后的存活率,可以通过添加在分离过程中丢失的ECM成分来改善胰岛微环境。此外,新的β细胞替代策略,如干细胞衍生β细胞(SCβC)治疗或替代移植部位和设备,可以从更好地了解β细胞如何与ECM相互作用中受益。在这篇小型综述中,我们讨论了目前对胰腺和胰岛ECM组成的理解,并综述了脱细胞方法,以产生用于胰岛和SCβC培养和移植的天然胰腺ECM支架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rebuilding a better home for transplanted islets.

Diabetes can be treated with β cell replacement therapy, where a patient is transplanted with cadaveric human islets to restore glycemic control. Despite this being an effective treatment, the process of isolating islets from the pancreas requires collagenase digestion which disrupts the islet extracellular matrix (ECM) and activates anoikis-mediated apoptosis. To improve islet survival in culture and after transplantation, the islet microenvironment may be enhanced with the addition of ECM components which are lost during isolation. Furthermore, novel β cell replacement strategies, such as stem cell-derived beta cell (SCβC) treatments or alternative transplant sites and devices, could benefit from a better understanding of how β cells interact with ECM. In this mini-review, we discuss the current understanding of the pancreas and islet ECM composition and review decellularization approaches to generate a native pancreatic ECM scaffold for use in both islet and SCβC culture and transplantation.

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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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