A New Method for Investigation of Different Tissues Using Femtosecond Laser Mass Spectrometry.

Background: Femtosecond laser mass spectrometry (FLMS) has become an important tool for investigation of chemical and biological materials in many areas from medical to industrial.

Objective: In medicine, the morphological examination of tissues is determined by performing pathological investigations under microscope. However, some novel improvements or developments must be performed for much faster diagnosis of the tissue during the operation when patient is under anesthesia.

Methods: The information obtained from the tissue under the microscope remains very limited because it cannot reveal characteristics of the whole molecules. For this reason, some novel methods for analysis of tissues are important issues to be achieved. This process can be performed using FLMS much quicker than traditional techniques. The aim of this study is to develop a new procedure for interpretation of mass spectra obtained from different types of muscle tissues, such as lamb, bones, and beef obtained from the butcher.

Results: The results obtained in this study are believed to open a new window for these kinds of applications for cancer diagnosis on human tissue studies, as a faster analysis technique to give some concrete contributions to pathological examinations. Both, principal component analysis statistical approach and FLMS technique offer a great opportunity to identify the biological materials from mass spectra. In conclusion, this present study interprets a great data from bone, beef, and lamb, which show that we can distinguish these different types of materials using FLMS data and statistical approaches.

Conclusions: Eventually, the experimental results obtained from our group studies present that these types of tissues can easily be distinguished using small m/q peaks in the lower region (m/z ≤100 amu) of the mass spectra by courtesy of FLMS.