开发用于筛选产生共轭亚油酸 (CLA) 的前列双歧杆菌菌株的快速方法。

Q2 Agricultural and Biological Sciences
Sun-Hae Choi, Kyoung-Min Lee, Kwan-Hu Kim, Geun-Bae Kim
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引用次数: 0

摘要

本研究旨在从新生儿粪便中分离出一些双歧杆菌菌株,并对其将亚油酸转化为共轭亚油酸(CLA)的生物转化活性进行筛选。研究人员采集了 20 名 14 至 100 天大的健康新生儿的粪便样本,并从双歧杆菌选择性反式寡糖培养基中随机选取了 400 个菌落。研究人员开发了一种双链聚合酶链式反应技术,用于快速准确地鉴定据报道具有产生 CLA 的物种特异性特征的双歧杆菌菌株。这些菌株通过 16S 核糖体 DNA、果糖-6-磷酸磷酸酮醇酶编码基因(xfp)和快速脉冲场凝胶电泳进行鉴定。36 个分离株被鉴定为布氏杆菌,12 个新生儿中只有两个携带布氏杆菌菌株。在分光光度测定法中,每个分离株都表现出不同的CLA产生能力。本研究中分离的菌株不同,它们的转化率也不同。根据产生 CLA 的活性,成功分离并鉴定了一些酒石酸杆菌菌株,有必要进行进一步研究,以鉴定酶和负责酶活性的基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of <i>Bifidobacterium breve</i>.

Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of <i>Bifidobacterium breve</i>.

Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of <i>Bifidobacterium breve</i>.

Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacterium breve.

This study was performed to isolate some strains of Bifidobacteriumbreve from fecal materials of neonates and to screen them for the biotransformation activity of converting linoleic acid into conjugated linoleic acid (CLA). Fecal samples were collected from twenty healthy neonates between 14 and 100 days old, and four hundred colonies were randomly selected from a Bifidobacterium selective transoligosaccharide medium. A duplex polymerase chain reaction technique was developed for the rapid and accurate molecular characterization of the B. breve strains that have been reported to show the species-specific characteristic of CLA production. They are identified by 16S ribosomal DNA, fructose-6-phosphate phosphoketolase encoding genes (xfp), and rapid pulsed field gel electrophoresis. Thirty-six isolates were identified as B. breve, and just two of the 12 neonates were harboring B. breve strains. Each isolate showed different CLA-producing ability in the spectrophotometric assay. All of the positive strains from the primary spectrophotometric assay were confirmed for their CLA-producing activities using gas-chromatographic analysis, and their conversion rates were different, depending on the strain isolated in this study. Some strains of B. breve were successfully isolated and characterized based on the CLA-producing activity, and further studies are necessary to characterize the enzyme and the gene responsible for the enzyme activity.

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来源期刊
CiteScore
1.22
自引率
0.00%
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审稿时长
4-8 weeks
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