[Genetic Characteristic Analysis of H Gene among Four Genotypes of MeasIes Viruses Isolated in Mainland China during 2013-2014].

To study the genetic characterization and amino acid mutation of hemagglutinin protein of four genotypes of wild type measles Viruses isolated in 2013 and 2014year,in China, including H1,B3,D8 and D9.Four genotype isolates of H1,D8,D9 and B3measles viruses were selected, and RNA of MV isolates were extracted. The complete sequence of hemagglutinin (1854nt) were amplified by RT-PCR and sequenced and Sequencher5.0was used to splice sequence. Phylogenetic analysis and the diversity of gene and amino acid were done by MEGA version 5.0,compared with 24 representative strains, repectively 4 H a sub-genotype strains,8D8 genotype strains,2D9 genotype strains,8B3 genotype strains and 2Chinese vaccine Strains, which were downloaded from GenBank. The homology of H gene nucleotide sequence and amino acid between the four genotype measles virus strains including H1,B3,D8,D9 and A genotype strains were respectively 94.2%-96.7%and 95.4%-96.7%and there were 20-28 amino acids difference between them; And the homology of H gene nucleotide sequence between Beijing14-1(H1a)and A genotype vaccine strains was 97.7%-98.2%.The N-glycosylation site in 240th was losen because of mutation. The homology of H gene nucleotide sequence and amino acid between the four genotype measles virus strains including H1,B3,D8,D9 and A genotype strains were high, and the Chinese vaccine can effectively prevent infection caused by H1asub-genotype,D8,D9 and B3imported genotypes virus strains.