C. Aguilera-Galvez , N. Champouret , H. Rietman , X. Lin , D. Wouters , Z. Chu , J.D.G. Jones , J.H. Vossen , R.G.F. Visser , P.J. Wolters , V.G.A.A. Vleeshouwers
{"title":"两个不同的R基因位点与晚疫病Avr2共同进化,赋予马铃薯不同的抗性特异性。","authors":"C. Aguilera-Galvez , N. Champouret , H. Rietman , X. Lin , D. Wouters , Z. Chu , J.D.G. Jones , J.H. Vossen , R.G.F. Visser , P.J. Wolters , V.G.A.A. Vleeshouwers","doi":"10.1016/j.simyco.2018.01.002","DOIUrl":null,"url":null,"abstract":"<div><p>Late blight, caused by the oomycete pathogen <em>Phytophthora infestans,</em> is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (<em>R</em>) genes. Such <em>R</em> genes against <em>P. infestans</em> have evolved in wild tuber-bearing <em>Solanum</em> species from North, Central and South America, upon co-evolution with cognate avirulence (<em>Avr</em>) genes. Here, we report how effectoromics screens with <em>Avr2</em> of <em>P. infestans</em> revealed defense responses in diverse <em>Solanum</em> species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican <em>Solanum</em> species is mediated by <em>R</em> genes of the <em>R2</em> family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian <em>Solanum</em> species is mediated by <em>Rpi-mcq1,</em> which resides on chromosome IX and does not belong to the <em>R2</em> family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian <em>Solanum</em> species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with <em>R</em> genes from either the <em>R2</em> or the <em>Rpi-mcq1</em> locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse <em>P. infestans</em> isolates. The achieved insights in the molecular <em>R – Avr</em> gene interaction can lead to more educated exploitation of <em>R</em> genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.</p></div>","PeriodicalId":22036,"journal":{"name":"Studies in Mycology","volume":"89 ","pages":"Pages 105-115"},"PeriodicalIF":14.1000,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.simyco.2018.01.002","citationCount":"31","resultStr":"{\"title\":\"Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato\",\"authors\":\"C. Aguilera-Galvez , N. Champouret , H. Rietman , X. Lin , D. Wouters , Z. Chu , J.D.G. Jones , J.H. Vossen , R.G.F. Visser , P.J. Wolters , V.G.A.A. Vleeshouwers\",\"doi\":\"10.1016/j.simyco.2018.01.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Late blight, caused by the oomycete pathogen <em>Phytophthora infestans,</em> is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (<em>R</em>) genes. Such <em>R</em> genes against <em>P. infestans</em> have evolved in wild tuber-bearing <em>Solanum</em> species from North, Central and South America, upon co-evolution with cognate avirulence (<em>Avr</em>) genes. Here, we report how effectoromics screens with <em>Avr2</em> of <em>P. infestans</em> revealed defense responses in diverse <em>Solanum</em> species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican <em>Solanum</em> species is mediated by <em>R</em> genes of the <em>R2</em> family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian <em>Solanum</em> species is mediated by <em>Rpi-mcq1,</em> which resides on chromosome IX and does not belong to the <em>R2</em> family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian <em>Solanum</em> species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with <em>R</em> genes from either the <em>R2</em> or the <em>Rpi-mcq1</em> locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse <em>P. infestans</em> isolates. The achieved insights in the molecular <em>R – Avr</em> gene interaction can lead to more educated exploitation of <em>R</em> genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.</p></div>\",\"PeriodicalId\":22036,\"journal\":{\"name\":\"Studies in Mycology\",\"volume\":\"89 \",\"pages\":\"Pages 105-115\"},\"PeriodicalIF\":14.1000,\"publicationDate\":\"2018-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.simyco.2018.01.002\",\"citationCount\":\"31\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Studies in Mycology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0166061618300022\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Studies in Mycology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166061618300022","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato
Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.
期刊介绍:
The international journal Studies in Mycology focuses on advancing the understanding of filamentous fungi, yeasts, and various aspects of mycology. It publishes comprehensive systematic monographs as well as topical issues covering a wide range of subjects including biotechnology, ecology, molecular biology, pathology, and systematics. This Open-Access journal offers unrestricted access to its content.
Each issue of Studies in Mycology consists of around 5 to 6 papers, either in the form of monographs or special focused topics. Unlike traditional length restrictions, the journal encourages submissions of manuscripts with a minimum of 50 A4 pages in print. This ensures a thorough exploration and presentation of the research findings, maximizing the depth of the published work.