两个不同的R基因位点与晚疫病Avr2共同进化,赋予马铃薯不同的抗性特异性。

IF 14.1 1区 生物学 Q1 MYCOLOGY
C. Aguilera-Galvez , N. Champouret , H. Rietman , X. Lin , D. Wouters , Z. Chu , J.D.G. Jones , J.H. Vossen , R.G.F. Visser , P.J. Wolters , V.G.A.A. Vleeshouwers
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引用次数: 31

摘要

晚疫病是由卵菌病原体晚疫病引起的马铃薯最具破坏性的病害。为了对这种经济上重要的疾病进行可持续管理,抗性育种依赖于抗性(R)基因的可用性。在与同源无毒性(Avr)基因共同进化的基础上,这种针对感染P.infestans的R基因在来自北美洲、中美洲和南美洲的野生块茎茄属物种中进化而来。在这里,我们报道了用感染P.infestans的Avr2进行的效应组学筛选如何揭示墨西哥和秘鲁原产的不同茄属物种的防御反应。我们发现,墨西哥茄属物种对AVR2的反应是由R2家族的R基因介导的,该基因位于染色体IV上的一个主要晚疫病基因座上。相反,秘鲁茄属物种的AVR2反应是由Rpi-mcq1介导的。数据表明,AVR2识别分别在墨西哥和秘鲁茄属物种的两个遗传位点上独立进化。对用R2或Rpi-mcq1基因座的R基因转化的马铃薯品种“Désirée”进行的分离叶试验显示,对18个不同的致病性疟原虫分离株的抗性谱重叠但不同。对分子R-Avr基因相互作用的深入了解可以使R基因得到更深入的利用,并最大限度地发挥产生更广谱、更持久地控制马铃薯晚疫病的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.

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来源期刊
Studies in Mycology
Studies in Mycology 生物-真菌学
CiteScore
35.60
自引率
3.00%
发文量
7
期刊介绍: The international journal Studies in Mycology focuses on advancing the understanding of filamentous fungi, yeasts, and various aspects of mycology. It publishes comprehensive systematic monographs as well as topical issues covering a wide range of subjects including biotechnology, ecology, molecular biology, pathology, and systematics. This Open-Access journal offers unrestricted access to its content. Each issue of Studies in Mycology consists of around 5 to 6 papers, either in the form of monographs or special focused topics. Unlike traditional length restrictions, the journal encourages submissions of manuscripts with a minimum of 50 A4 pages in print. This ensures a thorough exploration and presentation of the research findings, maximizing the depth of the published work.
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