大鼠肺用氢氧化钠非去污剂脱细胞液。

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-11 DOI:10.1080/15476278.2018.1462432
Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu
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引用次数: 24

摘要

肺移植是治疗终末期慢性肺疾病的最后选择。由于供体肺器官的短缺是主要障碍,肺再生被认为是克服这一障碍的方法。脱细胞器官支架的再细胞化是一种很有前途的器官再生方法。虽然去污剂通常用于脱细胞,但其他方法也是可能的。本研究采用高碱性(pH12)氢氧化钠(NaOH)-PBS无洗涤剂溶液进行肺脱细胞,并与以洗涤剂为基础的脱细胞溶液CHAPS和SDS对DNA去除和ECM保存的效果进行比较。免疫组织化学图像分析显示,细胞成分被NaOH溶液和其他洗涤剂去除。胶原蛋白和GAG分析显示,NaOH组的胶原蛋白减少量与CHAPS和SDS组相当。然而,NaOH组的DNA减少比其他组更显著(p < 0.0001)。HUVEC的再细胞化结果显示,其细胞附着力不逊于SDS组。体外功能分析显示,SDS组或NaOH组人工血红蛋白囊泡- pbs溶液通过再生肺时,100%氧通气使氧分压升高。结论是,基于NaOH-PBS的脱细胞溶液与普通脱细胞溶液相当,在成本效益和脱细胞支架残留物方面具有竞争力,从而为未来的临床使用提供了另一种潜在的洗涤剂选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sodium hydroxide based non-detergent decellularizing solution for rat lung.

Sodium hydroxide based non-detergent decellularizing solution for rat lung.

Sodium hydroxide based non-detergent decellularizing solution for rat lung.

Sodium hydroxide based non-detergent decellularizing solution for rat lung.

Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.

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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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