抗dfs抗体更常见于无因性静脉血栓栓塞患者。

Przeglad lekarski Pub Date : 2016-01-01
Joanna Natorska, Marta Mazur, Elżbieta Papuga-Szela, Anetta Undas
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To our knowledge there\nhave been no larger studies regarding\nthe prevalence of anti-DFS antibodies\nin provoked and unprovoked venous\nthromboembolism (VTE).</p><p><strong>Aim of the study: </strong>We investigated\nhow common anti-DFS70 antibodies\noccur in patients following VTE and\nwhich factors affect their occurrence\nin this disease.</p><p><strong>Material and methods: </strong>We screened\n287 consecutive adult patients, aged\nbelow 60 years, with documented\nVTE treated for at least 3 months, and\n129 age-matched healthy controls.\nPatients with cancer, severe comorbidities,\ndocumented autoimmune\ndiseases, including antiphospholipid\nsyndrome were ineligible. The anti-\n-DFS70 antibodies were determined\nbased on immunofluorescence on\nHep-2 cells. 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引用次数: 0

摘要

导语:据报道,在一小群患者中,有相当比例的抗dfs(致密细斑)抗体患者有特发性动脉或静脉血栓形成和/或产科并发症的病史。据我们所知,目前还没有关于抗dfs抗体诱发性和非诱发性静脉血栓栓塞(VTE)患病率的更大规模的研究。研究目的:我们调查了静脉血栓栓塞患者中常见的抗dfs70抗体是如何出现的,以及哪些因素影响了这种疾病的发生。材料和方法:我们筛选了287名年龄在60岁以下、dvte治疗至少3个月的连续成年患者,以及129名年龄匹配的健康对照。患有癌症、严重合并症、记录在案的自身免疫性疾病(包括抗磷脂综合征)的患者不符合条件。在hep -2细胞上用免疫荧光法检测抗DFS70抗体。在等于或大于1:100的血清稀释度下观察到的特异性免疫荧光模式,其特征是密集的细斑点分布在细胞核中,被认为是阳性的,并使用半定量elisa - dfs进行证实,该结果提供了比率(RU/ml),截止值为1。结果:静脉血栓栓塞患者与对照组的dfs70抗体阳性率无显著差异(n=12, 4.18% vs. n=6, 4.65%, p=0.68)。VTE患者抗dfs抗体占所有ANA阳性型的9.16% (n=131),远低于健康受试者的26.1% (p=0.031)。抗dfs抗体的存在与人口统计学或临床变量无关,包括自上次vtee事件以来的时间,抗凝类型及其质量。ana滴度≥1:20 20的患者中抗dfs70抗体的患病率高于滴度< 1:20 20的患者(75% vs. 37%, p=0.01)。重要的是,与诱发性VTE患者相比,非诱发性VTE患者中抗dfs抗体的患病率更高(75% vs. 25%, p=0.01)。在具有遗传性血栓形成的VTE患者中,即V - Leiden因子或凝血酶原G20210A突变,25.8%的受试者(n=8)有抗dfs抗体。此外,抗dfs滴度与血清α和γ球蛋白水平相关(r=0.47, p=0.027;r=0.39, p=0.045),但与VTE患者的炎症标志物或d -二聚体无关。结论:dfs抗体存在于
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-DFS antibodies occur more commonly in patients following unprovoked venous thromboembolism.

Introduction: It has been reported in a small group of patients that a significant percentage of patients with anti-DFS (dense fine speckled) antibodies has a history of idiopathic arterial or venous thrombosis and/or obstetric complications. To our knowledge there have been no larger studies regarding the prevalence of anti-DFS antibodies in provoked and unprovoked venous thromboembolism (VTE).

Aim of the study: We investigated how common anti-DFS70 antibodies occur in patients following VTE and which factors affect their occurrence in this disease.

Material and methods: We screened 287 consecutive adult patients, aged below 60 years, with documented VTE treated for at least 3 months, and 129 age-matched healthy controls. Patients with cancer, severe comorbidities, documented autoimmune diseases, including antiphospholipid syndrome were ineligible. The anti- -DFS70 antibodies were determined based on immunofluorescence on Hep-2 cells. The specific immunofluorescence pattern, characterized by dense fine speckles distributed the nucleus, observed at serum dilution equal to or greater than 1:100, was considered as positive and was confirmed using the semiquantitative ELISA-DFS that provided results as a ratio (RU/ml) with a cut-off of 1.

Results: There was no difference in the prevalence of anti-DFS70 antibodies in the VTE and control patients (n=12, 4.18% vs. n=6, 4.65%, p=0.68). Anti-DFS antibodies represented 9.16% of all positive ANA patterns (n=131) in VTE patients, which was a much lower proportion compared with 26.1% of all 23 positive ANA patterns in healthy subjects (p=0.031). The presence of anti-DFS antibodies did not correlate with demographic or clinical variables including time since last VTE event, type of anticoagulation and its quality. The prevalence of anti-DFS70 antibodies was higher in patients with ANA titer ≥1:320 compared to those with the titer < 1:320 (75% vs. 37%, p=0.01). Of importance, higher prevalence of anti-DFS antibodies was observed in patients with unprovoked VTE compared to those with provoked VTE (75% vs. 25%, p=0.01). Among the VTE patients with heritable thrombophilia, i.e. factor V Leiden or prothrombin G20210A mutations, 25.8% of subjects (n=8) had anti-DFS antibodies. Moreover, anti-DFS titer was associated with serum alpha and gamma globulin levels (r=0.47, p=0.027; and r=0.39, p=0.045, respectively), but not with inflammatory markers or D-dimer in VTE patients.

Conclusions: Anti-DFS antibodies are present in <5% of VTE patients and are associated with unprovoked VTE including that related to heritable thrombophilia. It might suggest that these antibodies are involved in the pathogenesis of idiopathic VTE.

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