光遗传学方法检测蛋白质-蛋白质相互作用的研究概况。

IF 7.9 Q1 Medicine
Matthew D Wiens, Robert E Campbell
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引用次数: 9

摘要

绘制蛋白质-蛋白质相互作用(PPi)景观对于进一步了解细胞和生物体的功能至关重要。光遗传学方法,即利用基因编码的荧光团或荧光酶反应的方法,独特地实现了活细胞中生物化学现象的可视化,具有很高的时空准确性。将光遗传学方法应用于PPis的检测需要基于蛋白质系统的工程设计,当两种感兴趣的蛋白质相互作用时,光信号会发生实质性的变化。近年来,研究人员已经开发了许多创造性和有效的光遗传学方法来实现这一目标,并利用它们进一步完善了我们的PPi景观图。在这篇综述中,我们介绍了光遗传学PPi检测的一般原理,然后提供了一些有代表性的例子,这些原理是如何应用的。我们根据产生的信号在本质上是可逆的还是不可逆的,以及信号在PPi的亚细胞部位是定位的还是非定位的,对方法进行了分类,组织了这篇综述。我们讨论了这些技术,给出了它们的优点和缺点,以便对它们的潜在用途做出理性的选择。本文分类如下:实验室方法与技术>成像实验室方法与技术>大分子相互作用,方法分析与计算方法>分析方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Surveying the landscape of optogenetic methods for detection of protein-protein interactions.

Surveying the landscape of optogenetic methods for detection of protein-protein interactions.

Surveying the landscape of optogenetic methods for detection of protein-protein interactions.

Mapping the protein-protein interaction (PPi) landscape is of critical importance to furthering our understanding how cells and organisms function. Optogenetic methods, that is, approaches that utilize genetically encoded fluorophores or fluorogenic enzyme reactions, uniquely enable the visualization of biochemical phenomena in live cells with high spatial and temporal accuracy. Applying optogenetic methods to the detection of PPis requires the engineering of protein-based systems in which an optical signal undergoes a substantial change when the two proteins of interest interact. In recent years, researchers have developed a number of creative and effective optogenetic methods that achieve this goal, and used them to further elaborate our map of the PPi landscape. In this review, we provide an introduction to the general principles of optogenetic PPi detection, and then provide a number of representative examples of how these principles have been applied. We have organized this review by categorizing methods based on whether the signal generated is reversible or irreversible in nature, and whether the signal is localized or nonlocalized at the subcellular site of the PPi. We discuss these techniques giving both their benefits and drawbacks to enable rational choices about their potential use. This article is categorized under: Laboratory Methods and Technologies > Imaging Laboratory Methods and Technologies > Macromolecular Interactions, Methods Analytical and Computational Methods > Analytical Methods.

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来源期刊
CiteScore
18.40
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Journal Name:Wiley Interdisciplinary Reviews-Systems Biology and Medicine Focus: Strong interdisciplinary focus Serves as an encyclopedic reference for systems biology research Conceptual Framework: Systems biology asserts the study of organisms as hierarchical systems or networks Individual biological components interact in complex ways within these systems Article Coverage: Discusses biology, methods, and models Spans systems from a few molecules to whole species Topical Coverage: Developmental Biology Physiology Biological Mechanisms Models of Systems, Properties, and Processes Laboratory Methods and Technologies Translational, Genomic, and Systems Medicine
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