工程基因组减少枯草芽孢杆菌从木糖生产乙酰素。

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2018-02-01 Epub Date: 2017-12-13 DOI:10.1007/s10529-017-2481-4

Panpan Yan, Yuanqing Wu, Li Yang, Zhiwen Wang, Tao Chen

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引用次数: 17

摘要

目的:研究一株基因组还原枯草芽孢杆菌作为基质细胞以木糖为原料生产乙酰素的能力。结果:为了使基因组减少的枯草芽孢杆菌BSK814具有利用木糖的能力，我们在其基因组中插入了一个天然的木基操纵子，并删除了araR基因。得到的菌株BSK814A2从10 g木糖/l中产生2.94 g乙酰素/l，比对照菌株BSK19A2高39%。bdhA和acoA基因的缺失进一步提高了BSK814A4对木糖的利用效率，使乙酰素产量提高到3.71 g/l。最后，BSK814A4从50 g木糖/l中产生23.3 g乙酰糖/l，产量为0.46 g/g木糖。其滴度和产量均比对照菌株BSK19A4高39%。结论:作为一个底盘细胞，基因组减少的枯草芽孢杆菌与野生型菌株相比，其从木糖中生产溢出产物乙酰素的能力显著提高。

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Engineering genome-reduced Bacillus subtilis for acetoin production from xylose.

Objectives: To investigate the capacity of a genome-reduced Bacillus subtilis strain as chassis cell for acetoin production from xylose.

Results: To endow the genome-reduced Bacillus subtilis strain BSK814 with the ability to utilize xylose, we inserted a native xyl operon into its genome and deleted the araR gene. The resulting strain BSK814A2 produced 2.94 g acetoin/l from 10 g xylose/l, which was 39% higher than control strain BSK19A2. The deletion of the bdhA and acoA genes further improved xylose utilization efficiency and increased acetoin production to 3.71 g/l in BSK814A4. Finally, BSK814A4 produced up to 23.3 g acetoin/l from 50 g xylose/l, with a yield of 0.46 g/g xylose. Both the titer and yield were 39% higher than those of control strain BSK19A4.

Conclusions: As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain.

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.