利用微pet /CT纵向评价空腹和非空腹自发性高血压大鼠心肌脂肪酸和葡萄糖代谢。

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2017-01-01 DOI:10.1177/1536012117724558

Jennifer S Huber, Andrew M Hernandez, Mustafa Janabi, James P O'Neil, Kathleen M Brennan, Stephanie T Murphy, Youngho Seo, Grant T Gullberg

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引用次数: 6

摘要

通过纵向微正电子发射断层扫描(microPET)/计算机断层扫描(CT)研究，我们量化了自发性高血压大鼠(SHRs)心肌代谢和灌注的变化，这是一种左心室肥厚(LVH)模型。采用长链脂肪酸类似物18f -氟-6-硫-十六烷酸(18F-FTHA)和葡萄糖类似物18f -氟脱氧葡萄糖(18F-FDG)，在正常或空腹条件下定量测定SHRs和Wistar-Kyoto (WKY)正常血压大鼠心脏的脂肪酸和葡萄糖代谢。我们还使用18f -氟二氢烯醇(18F-FDHROL)在不禁食的情况下观察心脏灌注情况。在大鼠的整个生命周期中对其进行4到5次成像。用隔室模型估计放射性示踪剂的速率常数。采集血液样本，分析葡萄糖和游离脂肪酸浓度。在2009-2010研究期间，无禁食的SHRs在18F-FDHROL洗入速率常数(P = 0.1)和分布体积(P = 0.1)方面与WKYs无显著差异，18F-FDG心肌内流速率常数显著高于WKYs (P = 4×10-8)， 18F-FTHA心肌内流速率常数显著低于WKYs (P = 0.007)。在2011-2012年空腹研究中，SHRs的18F-FDHROL洗入速率常数(P = 5×10-6)和分布体积(P = 3×10-8)显著高于WKYs, 18F-FDG心肌内流速率常数(P = 3×10-8)显著高于WKYs, 18F-FTHA心肌内流速率常数的趋势(P = 0.1，无统计学意义)高于WKYs。葡萄糖血浆浓度的变化通常与相应的放射性示踪剂内流速率常数变化呈负相关。该研究表明，从首选脂肪酸代谢到增加葡萄糖代谢与肥厚的转换。2011-2012年研究期间灌注增加可能表明LVH SHR模型中有氧代谢增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Longitudinal Evaluation of Myocardial Fatty Acid and Glucose Metabolism in Fasted and Nonfasted Spontaneously Hypertensive Rats Using MicroPET/CT.

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Longitudinal Evaluation of Myocardial Fatty Acid and Glucose Metabolism in Fasted and Nonfasted Spontaneously Hypertensive Rats Using MicroPET/CT.

Using longitudinal micro positron emission tomography (microPET)/computed tomography (CT) studies, we quantified changes in myocardial metabolism and perfusion in spontaneously hypertensive rats (SHRs), a model of left ventricular hypertrophy (LVH). Fatty acid and glucose metabolism were quantified in the hearts of SHRs and Wistar-Kyoto (WKY) normotensive rats using long-chain fatty acid analog ¹⁸F-fluoro-6-thia heptadecanoic acid (¹⁸F-FTHA) and glucose analog ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG) under normal or fasting conditions. We also used ¹⁸F-fluorodihydrorotenol (¹⁸F-FDHROL) to investigate perfusion in their hearts without fasting. Rats were imaged at 4 or 5 times over their life cycle. Compartment modeling was used to estimate the rate constants for the radiotracers. Blood samples were obtained and analyzed for glucose and free fatty acid concentrations. SHRs demonstrated no significant difference in ¹⁸F-FDHROL wash-in rate constant ( P = .1) and distribution volume ( P = .1), significantly higher ¹⁸F-FDG myocardial influx rate constant ( P = 4×10^-8), and significantly lower ¹⁸F-FTHA myocardial influx rate constant ( P = .007) than WKYs during the 2009-2010 study without fasting. SHRs demonstrated a significantly higher ¹⁸F-FDHROL wash-in rate constant ( P = 5×10^-6) and distribution volume ( P = 3×10^-8), significantly higher ¹⁸F-FDG myocardial influx rate constant ( P = 3×10^-8), and a higher trend of ¹⁸F-FTHA myocardial influx rate constant (not significant, P = .1) than WKYs during the 2011-2012 study with fasting. Changes in glucose plasma concentrations were generally negatively correlated with corresponding radiotracer influx rate constant changes. The study indicates a switch from preferred fatty acid metabolism to increased glucose metabolism with hypertrophy. Increased perfusion during the 2011-2012 study may be indicative of increased aerobic metabolism in the SHR model of LVH.

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.