荧光/发光法测定细胞模型中植物化学物质的抗氧化活性。

Izabela Koss-Mikołajczyk, Monika Baranowska, Jacek Namieśnik, Agnieszka Bartoszek
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引用次数: 10

摘要

一旦发现活性氧(ROS)在所谓的文明疾病(包括癌症、糖尿病或高血压等非传染性慢性疾病)中的作用,并提出使用抗氧化剂作为这些疾病的治疗方法的可能性,科学家们就开发了广泛的方法来确定纯化学品和植物提取物以及膳食补充剂的抗氧化活性。这些方法大多基于抗氧化剂和ROS之间的简单氧化还原反应(例如ABTS, DPPH或FRAP测试)。然而,评估抗氧化活性的化学方法很少具有生物学意义。它们并不能反映抗氧化剂在生物体中的真实作用,因为它们是在温度和pH等非生理条件下使用的;它们既不考虑代谢也不考虑细胞内运输。评估生物体抗氧化活性的最佳模型是人体或动物模型,但这类模型的测定非常复杂且往往模棱两可。目前化学和人体试验的最佳替代方法是使用细胞培养模型的分析,它比人体试验便宜,但仍然比化学分析更能令人信服地反映生物系统。细胞抗氧化测定是在生理pH值和温度下进行的,但最重要的是,它们考虑了代谢和细胞内运输。在这篇综述中,我们介绍了用于测定抗氧化活性的基于发光和荧光方法的细胞测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of antioxidantactivity of phytochemicals in cellular models by fluorescence/luminescence methods.

As soon as the role of Reactive Oxygen Species (ROS) in so-called civilization diseases, which include non-infectious chronic diseases such as cancer, diabetes or high blood pressure has been discovered, and the possibility of employing antioxidants as a remedy for these diseases have been proposed, scientists developed a broad spectrum of methods to determine antioxidant activity of pure chemicals and plant extracts, as well as dietary supplements. Most of these methods are based on simple redox reactions between antioxidant and ROS (for example ABTS, DPPH, or FRAP tests). However, chemical methods of assessing antioxidant activity are rarely biologically relevant. They do not mirror the real effect of antioxidants in living organisms, because they are used in non-physiological conditions of temperature and pH; neither they take metabolism nor intracellular transport under consideration. The perfect model for assessment of antioxidant activity in living organisms would be human or animal model, but such determinations are very complicated and often ambiguous. The current best alternative to chemical and human tests are assays employing cell culture models being less expensive than human tests, yet still reflecting biological systems more convincingly than chemical assays. Cellular antioxidant assays are performed under physiological pH and temperature, but most importantly, they take metabolism and intracellular transport under consideration. In this review, we present cellular tests used to determine antioxidant activity that are based on luminescence and fluorescence methods.

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