大肠杆菌亲环蛋白PpiA与生物膜形成的结构-功能分析。

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology
Aggeliki Skagia, Eleni Vezyri, Konstantinos Grados, Anastasia Venieraki, Michael Karpusas, Panagiotis Katinakis, Maria Dimou
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引用次数: 3

摘要

肽基-丙氨酸顺式/反式异构酶(PPIases, EC: 5.2.1.8)在生命的所有领域的存在表明了它们的生物学重要性。亲环蛋白PpiA存在于革兰氏阴性菌的周质中,具有PPIase活性,但其生理功能尚不明确。在这里,我们证明了与野生型菌株相比,来自大肠杆菌的ΔppiA缺失菌株表现出更强的生物膜形成能力和更强的游泳运动能力。为了确定PpiA对生物膜形成的负调节所必需的结构特征,我们使用易出错和位点定向诱变方法的组合构建了一系列突变的PpiA蛋白。我们发现PpiA对生物膜形成的负面影响并不取决于其PPIase活性,因为PPIase活性降低的PpiA突变体能够在生物膜生长过程中补充ΔppiA菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation.

The presence of peptidyl-prolyl cis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functions are still not clearly defined. Here, we demonstrate that the ΔppiA deletion strain from Escherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mutant PpiA proteins using a combination of error-prone and site-directed mutagenesis approaches. We show that the negative effect of PpiA on biofilm formation is not dependent on its PPIase activity, since PpiA mutants with a reduced PPIase activity are able to complement the ΔppiA strain during biofilm growth.

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来源期刊
Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物
CiteScore
3.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.
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