{"title":"光甘草定对石川细胞雌激素应答基因的DNA芯片分析。","authors":"Poh Su Wei Melissa, Yong Voon Chen Phelim, Visweswaran Navaratnam, Chia Yoke Yin","doi":"10.1177/1178626417721676","DOIUrl":null,"url":null,"abstract":"<p><p>Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E<sub>2</sub>, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E<sub>2</sub> in comparison with 17β-E<sub>2</sub>. The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E<sub>2</sub> regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E<sub>2</sub> seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin's function as a phytoestrogen and suggested new ideas for the usage of glabridin.</p>","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1178626417721676","citationCount":"4","resultStr":"{\"title\":\"DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin.\",\"authors\":\"Poh Su Wei Melissa, Yong Voon Chen Phelim, Visweswaran Navaratnam, Chia Yoke Yin\",\"doi\":\"10.1177/1178626417721676\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E<sub>2</sub>, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E<sub>2</sub> in comparison with 17β-E<sub>2</sub>. The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E<sub>2</sub> regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E<sub>2</sub> seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin's function as a phytoestrogen and suggested new ideas for the usage of glabridin.</p>\",\"PeriodicalId\":8791,\"journal\":{\"name\":\"Biochemistry Insights\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1177/1178626417721676\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/1178626417721676\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/1178626417721676","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin.
Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E2, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E2 in comparison with 17β-E2. The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E2 regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E2 seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin's function as a phytoestrogen and suggested new ideas for the usage of glabridin.
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