使用新型广靶向合成核蛋白抗原阵列对北欧汉坦病毒进行血清分型和血清流行病学研究。

Q1 Environmental Science
Infection Ecology and Epidemiology Pub Date : 2017-07-26 eCollection Date: 2017-01-01 DOI:10.1080/20008686.2017.1350086
Bengt Rönnberg, Olli Vapalahti, Marco Goeijenbier, Chantal Reusken, Åke Gustafsson, Jonas Blomberg, Åke Lundkvist
{"title":"使用新型广靶向合成核蛋白抗原阵列对北欧汉坦病毒进行血清分型和血清流行病学研究。","authors":"Bengt Rönnberg,&nbsp;Olli Vapalahti,&nbsp;Marco Goeijenbier,&nbsp;Chantal Reusken,&nbsp;Åke Gustafsson,&nbsp;Jonas Blomberg,&nbsp;Åke Lundkvist","doi":"10.1080/20008686.2017.1350086","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction</b>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. <b>Materials and methods</b>: 'Megapeptides', 119-120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT). <b>Results and discussion</b>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations. <b>Conclusion</b>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"7 1","pages":"1350086"},"PeriodicalIF":0.0000,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2017.1350086","citationCount":"3","resultStr":"{\"title\":\"Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.\",\"authors\":\"Bengt Rönnberg,&nbsp;Olli Vapalahti,&nbsp;Marco Goeijenbier,&nbsp;Chantal Reusken,&nbsp;Åke Gustafsson,&nbsp;Jonas Blomberg,&nbsp;Åke Lundkvist\",\"doi\":\"10.1080/20008686.2017.1350086\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction</b>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. <b>Materials and methods</b>: 'Megapeptides', 119-120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT). <b>Results and discussion</b>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations. <b>Conclusion</b>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>\",\"PeriodicalId\":37446,\"journal\":{\"name\":\"Infection Ecology and Epidemiology\",\"volume\":\"7 1\",\"pages\":\"1350086\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/20008686.2017.1350086\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Ecology and Epidemiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/20008686.2017.1350086\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Ecology and Epidemiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/20008686.2017.1350086","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 3

摘要

汉坦病毒是全球分布的人畜共患病原体。其多样性和高的抗原交叉反应性使得传统的诊断方法十分繁琐。材料和方法:利用16种汉坦病毒核蛋白氨基端119-120米的“巨肽”,代表了汉坦病毒系统发育树的四个主要分支,在一种新的基于igg的汉坦病毒悬浊液多重免疫测定(HSMIA)中检测155个北欧人样本中过去的汉坦病毒感染。我们将HSMIA与既定的eia和焦点减少中和试验(FRNT)进行比较。结果与讨论:Puumala汉坦病毒(PUUV)组件在HSMIA给整合结果PUUV免疫球蛋白g 142年环境影响评价血清从瑞典北部(31环评是积极的,7边缘型和104年环境影响评价消极,灵敏度30/31 = 97%,特异性104/104 = 100%,134/135 = 99%的一致性),与另一个免疫测定40 PUUV免疫球蛋白阳性血清从芬兰灵敏度(36/40 = 90%),并整合8 11例PUUV DOBV中和滴度,分别。发现两种主要的IgG反应模式:(i) puuv特异性模式覆盖系统群IV及其血清群B和C;(ii)杜布拉瓦病毒(DOBV)特异性模式,覆盖种系群III的血清组a部分。此外,我们还发现了几种仅对一个或两个巨肽具有反应性的小模式,这表明在瑞典和芬兰人群中还有其他汉坦病毒感染人类。结论:广泛反应和合理的HSMIA产生的结果与建立的PUUV eia和NT结果高度相关。这是一种灵敏、特异的检测方法,适用于人类汉坦病毒的高效血清监测。它在动物身上的应用还有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.

Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.

Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.

Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.

Introduction: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. Materials and methods: 'Megapeptides', 119-120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT). Results and discussion: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations. Conclusion: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Infection Ecology and Epidemiology
Infection Ecology and Epidemiology Environmental Science-Environmental Science (miscellaneous)
CiteScore
8.70
自引率
0.00%
发文量
4
审稿时长
12 weeks
期刊介绍: Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信