返回瑞典的旅行者患非洲蜱叮咬热。报告两个病例及其诊断方面的情况。

Q1 Environmental Science
Infection Ecology and Epidemiology Pub Date : 2017-08-02 eCollection Date: 2017-01-01 DOI:10.1080/20008686.2017.1343081
Kenneth Nilsson, Katarina Wallménius, Pernilla Rundlöf-Nygren, Susanne Strömdahl, Carl Påhlson
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引用次数: 13

摘要

导言:由非洲立克次体引起的非洲蜱叮咬热是撒哈拉以南非洲农村地区的一种地方性疾病,也是回国的瑞典旅行者发烧的可能原因。本文介绍了两例患者,并讨论了不同诊断方法的优缺点。患者与方法:两名中年男子从南非回国后出现发热症状。两例均为单发疮痂,其中一例还伴有淋巴结肿胀。使用拭子从伤口(患者1)采集样本进行血清学和一般细菌培养,并对患者2的痂皮活检进行PCR检测。结果和讨论:两例患者发病后1个月血清转化。活体组织的实时PCR结果为阳性,gltA基因测序结果与非洲致病菌的一致性为99-100%。从活检中提取的一滴液体含有足够数量的细菌,也允许在Vero细胞培养中分离立克次体。对患者1的疮痂进行细菌培养的拭子进行直接分子PCR检测也产生了阳性结果。总之,研究结果强调了拭子对发热旅行者非洲蜱叮咬热的早期非侵入性诊断的有用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

African tick bite fever in returning Swedish travellers. Report of two cases and aspects of diagnostics.

African tick bite fever in returning Swedish travellers. Report of two cases and aspects of diagnostics.

African tick bite fever in returning Swedish travellers. Report of two cases and aspects of diagnostics.

Introduction: African tick-bite fever, caused by Rickettsia africae, is endemic in rural areas of sub-Saharan Africa and a possible cause of fever in returning Swedish travellers. Two patients are presented, and the advantages and disadvantages of different diagnostic methods are discussed. Patients and methods: Two middle-aged men fell ill with fever after returning home from South Africa. Both had single eschars and one also presented with a lymph node swelling. Samples were taken for serology, general bacterial culture from the wound (Patient 1) using a swab and additionally for Patient 2 PCR of a skin biopsy from the eschar. Results and discussion: Both patients seroconverted one month after onset. Real-time PCR of the biopsy was positive, where sequencing of the gltA gene was 99-100% consistent with R. africae. A drop of fluid from the biopsy contained a sufficient number of bacteria to also allow for isolation of rickettsiae in Vero cell culture. Direct molecular detection by PCR from a swab used for bacteria culture from the eschar from Patient 1 also yielded a positive result. In conclusion, the findings highlight the usefulness of swabs for early non-invasive diagnosis of African tick-bite fever in febrile travellers.

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来源期刊
Infection Ecology and Epidemiology
Infection Ecology and Epidemiology Environmental Science-Environmental Science (miscellaneous)
CiteScore
8.70
自引率
0.00%
发文量
4
审稿时长
12 weeks
期刊介绍: Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment
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