Kenneth Nilsson, Katarina Wallménius, Pernilla Rundlöf-Nygren, Susanne Strömdahl, Carl Påhlson
{"title":"返回瑞典的旅行者患非洲蜱叮咬热。报告两个病例及其诊断方面的情况。","authors":"Kenneth Nilsson, Katarina Wallménius, Pernilla Rundlöf-Nygren, Susanne Strömdahl, Carl Påhlson","doi":"10.1080/20008686.2017.1343081","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction</b>: African tick-bite fever, caused by <i>Rickettsia africae</i>, is endemic in rural areas of sub-Saharan Africa and a possible cause of fever in returning Swedish travellers. Two patients are presented, and the advantages and disadvantages of different diagnostic methods are discussed. <b>Patients and methods</b>: Two middle-aged men fell ill with fever after returning home from South Africa. Both had single eschars and one also presented with a lymph node swelling. Samples were taken for serology, general bacterial culture from the wound (Patient 1) using a swab and additionally for Patient 2 PCR of a skin biopsy from the eschar. <b>Results and discussion</b>: Both patients seroconverted one month after onset. Real-time PCR of the biopsy was positive, where sequencing of the <i>gltA</i> gene was 99-100% consistent with <i>R. africae</i>. A drop of fluid from the biopsy contained a sufficient number of bacteria to also allow for isolation of rickettsiae in Vero cell culture. Direct molecular detection by PCR from a swab used for bacteria culture from the eschar from Patient 1 also yielded a positive result. In conclusion, the findings highlight the usefulness of swabs for early non-invasive diagnosis of African tick-bite fever in febrile travellers.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"7 1","pages":"1343081"},"PeriodicalIF":0.0000,"publicationDate":"2017-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2017.1343081","citationCount":"13","resultStr":"{\"title\":\"African tick bite fever in returning Swedish travellers. Report of two cases and aspects of diagnostics.\",\"authors\":\"Kenneth Nilsson, Katarina Wallménius, Pernilla Rundlöf-Nygren, Susanne Strömdahl, Carl Påhlson\",\"doi\":\"10.1080/20008686.2017.1343081\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction</b>: African tick-bite fever, caused by <i>Rickettsia africae</i>, is endemic in rural areas of sub-Saharan Africa and a possible cause of fever in returning Swedish travellers. Two patients are presented, and the advantages and disadvantages of different diagnostic methods are discussed. <b>Patients and methods</b>: Two middle-aged men fell ill with fever after returning home from South Africa. Both had single eschars and one also presented with a lymph node swelling. Samples were taken for serology, general bacterial culture from the wound (Patient 1) using a swab and additionally for Patient 2 PCR of a skin biopsy from the eschar. <b>Results and discussion</b>: Both patients seroconverted one month after onset. Real-time PCR of the biopsy was positive, where sequencing of the <i>gltA</i> gene was 99-100% consistent with <i>R. africae</i>. A drop of fluid from the biopsy contained a sufficient number of bacteria to also allow for isolation of rickettsiae in Vero cell culture. Direct molecular detection by PCR from a swab used for bacteria culture from the eschar from Patient 1 also yielded a positive result. In conclusion, the findings highlight the usefulness of swabs for early non-invasive diagnosis of African tick-bite fever in febrile travellers.</p>\",\"PeriodicalId\":37446,\"journal\":{\"name\":\"Infection Ecology and Epidemiology\",\"volume\":\"7 1\",\"pages\":\"1343081\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/20008686.2017.1343081\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Ecology and Epidemiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/20008686.2017.1343081\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Ecology and Epidemiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/20008686.2017.1343081","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}
African tick bite fever in returning Swedish travellers. Report of two cases and aspects of diagnostics.
Introduction: African tick-bite fever, caused by Rickettsia africae, is endemic in rural areas of sub-Saharan Africa and a possible cause of fever in returning Swedish travellers. Two patients are presented, and the advantages and disadvantages of different diagnostic methods are discussed. Patients and methods: Two middle-aged men fell ill with fever after returning home from South Africa. Both had single eschars and one also presented with a lymph node swelling. Samples were taken for serology, general bacterial culture from the wound (Patient 1) using a swab and additionally for Patient 2 PCR of a skin biopsy from the eschar. Results and discussion: Both patients seroconverted one month after onset. Real-time PCR of the biopsy was positive, where sequencing of the gltA gene was 99-100% consistent with R. africae. A drop of fluid from the biopsy contained a sufficient number of bacteria to also allow for isolation of rickettsiae in Vero cell culture. Direct molecular detection by PCR from a swab used for bacteria culture from the eschar from Patient 1 also yielded a positive result. In conclusion, the findings highlight the usefulness of swabs for early non-invasive diagnosis of African tick-bite fever in febrile travellers.
期刊介绍:
Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment