纳米银对铜绿假单胞菌的抑菌作用。

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2017-06-29 eCollection Date: 2017-01-01 DOI:10.2147/NSA.S133415
R Salomoni, P Léo, A F Montemor, B G Rinaldi, Mfa Rodrigues
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引用次数: 205

摘要

铜绿假单胞菌具有很强的内在耐药性,限制了有效抗生素的数量。因此,其他抗菌剂,如银纳米颗粒(AgNPs)被认为是帮助管理和预防感染的潜在药物。AgNPs可用于多种应用,以对抗对常见抗生素耐药的细菌,甚至多重耐药细菌,如铜绿假单胞菌。本研究评估了商用10 nm AgNPs对两株对大量抗生素耐药的铜绿假单胞菌医院菌株和一株培养收集的参考菌株的抗菌活性。所有菌株对5µg/mL纳米颗粒溶液均敏感。对照菌株INCQS 0230和P.a.1分别在浓度为1.25和0.156µg/mL时对AgNPs敏感;然而,在医院菌株P.a.2中没有观察到这种情况,该菌株对所有抗生素和AgNPs都具有更强的耐药性。细胞毒性评价表明,AgNPs浓度达到2.5µg/mL时,对所有被测细胞系都是非常安全的。在5.0µg/mL浓度下,AgNPs对肿瘤细胞HeLa和HepG2具有离散的细胞毒性作用。结果表明,AgNPs具有替代现有常规抗菌剂的潜力,并为纳米银与抗生素结合应用增强抗菌活性提供了前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antibacterial effect of silver nanoparticles in <i>Pseudomonas aeruginosa</i>.

Antibacterial effect of silver nanoparticles in <i>Pseudomonas aeruginosa</i>.

Antibacterial effect of silver nanoparticles in <i>Pseudomonas aeruginosa</i>.

Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa.

Pseudomonas aeruginosa has great intrinsic antimicrobial resistance limiting the number of effective antibiotics. Thus, other antimicrobial agents such as silver nanoparticles (AgNPs) are considered potential agents to help manage and prevent infections. AgNPs can be used in several applications against bacteria resistant to common antibiotics or even multi-resistant bacteria such as P. aeruginosa. This study assessed the antimicrobial activity of commercial 10 nm AgNPs on two hospital strains of P. aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection. All strains were susceptible to 5 µg/mL nanoparticles solution. Reference strains INCQS 0230 and P.a.1 were sensitive to AgNPs at concentrations of 1.25 and 0.156 µg/mL, respectively; however, this was not observed for hospital strain P.a.2, which was more resistant to all antibiotics and AgNPs tested. Cytotoxicity evaluation indicated that AgNPs, up to a concentration of 2.5 µg/mL, are very safe for all cell lines tested. At 5.0 µg/mL, AgNPs had a discrete cytotoxic effect on tumor cells HeLa and HepG2. Results showed the potential of using AgNPs as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity.

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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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