果蝇突触对中dprp - dip匹配表达。

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fly Pub Date : 2017-01-02 Epub Date: 2016-07-22 DOI:10.1080/19336934.2016.1214784
Marta Morey
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引用次数: 2

摘要

神经元形成了精确的连接模式。调控突触伴侣选择的细胞识别机制尚不清楚。作为细胞-细胞相互作用的最终介质,细胞表面和分泌分子(csm)在这一过程中发挥着重要作用。为了深入了解神经元如何区分突触伙伴,我们分析了在果蝇视觉系统的髓质神经层中形成不同突触连接的7个密切相关的神经元的转录组。我们的测序数据显示,这些神经元中的每一个在突触形成时都表达了数百个csm的独特组合。研究人员发现,21个缺陷长鼻延伸反应(Dpr)家族的类似物以独特的细胞类型特异性方式表达,与所描述的每个神经元的独特连接模式一致。对它们的同源结合伙伴Dpr相互作用蛋白(DIP)家族的9个成员的表达分析显示,髓质中存在互补的层特异性表达,表明表达Dpr的神经元与表达同一层DIP的神经元之间存在相互作用。通过共表达分析和连接组数据的相关性,我们将表达DIP的神经元识别为表达Dpr神经元的突触伴侣的一个子集。我们提出dprp - dip相互作用调节表达它们的神经元之间的连接模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dpr-DIP matching expression in Drosophila synaptic pairs.

Dpr-DIP matching expression in Drosophila synaptic pairs.

Neurons form precise patterns of connections. The cellular recognition mechanisms regulating the selection of synaptic partners are poorly understood. As final mediators of cell-cell interactions, cell surface and secreted molecules (CSMs) are expected to play important roles in this process. To gain insight into how neurons discriminate synaptic partners, we profiled the transcriptomes of 7 closely related neurons forming distinct synaptic connections in discrete layers in the medulla neuropil of the fly visual system. Our sequencing data revealed that each one of these neurons expresses a unique combination of hundreds of CSMs at the onset of synapse formation. We show that 21 paralogs of the defective proboscis extension response (Dpr) family are expressed in a unique cell-type-specific fashion, consistent with the distinct connectivity pattern of each neuron profiled. Expression analysis of their cognate binding partners, the 9 members of the Dpr interacting protein (DIP) family, revealed complementary layer-specific expression in the medulla, suggestive of interactions between neurons expressing Dpr and those expressing DIP in the same layer. Through coexpression analysis and correlation to connectome data, we identify neurons expressing DIP as a subset of the synaptic partners of the neurons expressing Dpr. We propose that Dpr-DIP interactions regulate patterns of connectivity between the neurons expressing them.

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来源期刊
Fly
Fly 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.
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