IGF-2、IGF-1R、IGF-2R和PTEN参与人牙胚发育的免疫组织化学研究

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2016-07-02 Epub Date: 2016-06-21 DOI:10.1080/15476278.2016.1197460
Darko Kero, Livia Cigic, Ivana Medvedec Mikic, Tea Galic, Mladen Cubela, Katarina Vukojevic, Mirna Saraga-Babic
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引用次数: 14

摘要

胰岛素样生长因子2 (IGF-2)是一种对产前生长发育至关重要的肽激素。IGF-2通过胰岛素样生长因子1受体(IGF-1R)发挥有丝分裂作用,并通过与胰岛素样生长受体2 (IGF-2R)结合而消除。IGF-2也受到磷酸酶和紧张素同源物(PTEN)的负调控,PTEN是一种在多种肿瘤中突变的磷酸酶。在人类牙形成过程中,这些因素之间的相互作用尚不清楚。本研究采用双免疫荧光法分析了7 ~ 20孕周胎儿期人门牙胚中IGF-2、IGF-1R、IGF-2R和PTEN的表达规律。在研究期间,IGF-2主要在牙釉质器官中表达,而PTEN在牙乳头和部分牙釉质器官中轻度至中度表达。IGF-1R在整个牙釉质器官中普遍表达,且表达强度高。相比之下,IGF-2R在牙釉质器官和牙乳头中的表达都不稳定。IGF-2、IGF-1R、IGF-2R和PTEN在高增殖颈袢以及牙釉质器官形成的钟形早期和晚钟形阶段尖尖区分化前成釉细胞和前成牙细胞中的表达模式,表明这些因素在人门牙冠形态发生中的重要性。总之,我们的数据表明,在正常牙齿发育过程中,IGF-2、IGF-1R、IGF-2R和PTEN参与了基本细胞过程(增殖、分化)的时空模式。它们也与提高对人类牙形成分子基础的认识有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Involvement of IGF-2, IGF-1R, IGF-2R and PTEN in development of human tooth germ - an immunohistochemical study.

Involvement of IGF-2, IGF-1R, IGF-2R and PTEN in development of human tooth germ - an immunohistochemical study.

Involvement of IGF-2, IGF-1R, IGF-2R and PTEN in development of human tooth germ - an immunohistochemical study.

Involvement of IGF-2, IGF-1R, IGF-2R and PTEN in development of human tooth germ - an immunohistochemical study.

Insulin-Like Growth Factor 2 (IGF-2) is a peptide hormone essential for prenatal growth and development. IGF-2 exerts its mitogenic effects via Insulin-Like Growth Factor 1 Receptor (IGF-1R), and is eliminated by binding to Insulin-Like Growth Receptor 2 (IGF-2R). IGF-2 is also negatively regulated by Phosphatase and Tensin Homolog (PTEN), a phosphatase mutated in various tumors. Not much is known about the interplay between these factors during human odontogenesis. In this study, expression patterns of IGF-2, IGF-1R, IGF-2R and PTEN were analyzed by double immunofluorescence in incisor human tooth germs during the foetal period of development between the 7th and 20th gestational week. Throughout the investigated period, IGF-2 was mostly expressed in enamel organ, whereas mild to moderate expression of PTEN could be seen in dental papilla and parts of enamel organ. Expression of IGF-1R was ubiquitous and displayed strong intensity throughout the entire enamel organ. In contrast, expression of IGF-2R had rather erratic pattern in enamel organ and dental papilla alike. Expression patterns of IGF-2, IGF-1R, IGF-2R and PTEN in highly proliferative cervical loops, as well as in differentiating pre-ameloblasts and pre-odontoblasts of cusp tip region during the early and late bell stages when enamel organ acquires definitive shape, indicate importance of these factors in crown morphogenesis of human incisor. Taken together, our data suggest the involvement of IGF-2, IGF-1R, IGF-2R and PTEN in temporo-spatial patterning of basic cellular processes (proliferation, differentiation) during normal tooth development. They are also relevant for improving knowledge of molecular basis of human odontogenesis.

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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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