黑曲霉的转录激活剂GaaR是果胶中d-半乳糖醛酸释放和利用的必需物质

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2016-05-13 DOI:10.1002/1873-3468.12211

Ebru Alazi, Jing Niu, Joanna E. Kowalczyk, Mao Peng, Maria Victoria Aguilar Pontes, Jan A. L. van Kan, Jaap Visser, Ronald P. de Vries, Arthur F. J. Ram

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引用次数: 58

摘要

我们鉴定了腐坏性真菌黑曲霉(Aspergillus niger)的d-半乳糖醛酸(GA)应答转录激活子GaaR，该转录激活子对GA和聚半乳糖醛酸(PGA)的生长至关重要。在复杂的果胶上，ΔgaaR菌株的生长受到抑制。全基因组表达分析表明，GaaR是表达从PGA和更复杂的果胶中释放GA、将GA转运到细胞中以及诱导GA分解代谢途径所需的基因所必需的。ΔgaaR在复合果胶上的残留生长可能是由于果胶酶的表达作用于鼠李糖半乳果糖酸，以及随后对除GA以外的单糖的代谢。

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The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d-galacturonic acid from pectin

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The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d-galacturonic acid from pectin

We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.