人肋关节软骨细胞膜通道基因的表达。

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2016-04-02 Epub Date: 2016-04-26 DOI:10.1080/15476278.2016.1181238
A Asmar, R Barrett-Jolley, A Werner, R Kelly, M Stacey
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引用次数: 21

摘要

软骨细胞是所有类型软骨中唯一的常驻细胞,其功能的关键是通过代谢活性的变化来响应机械负荷的能力。这种机械转导特性在一定程度上是通过一系列表达的跨膜通道的活性介导的;离子通道、间隙连接蛋白和孔蛋白。离子通道的适当表达已被证明对细胞外基质的产生至关重要,跨膜通道的差异表达与骨关节炎和Albers-Schönberg等肌肉骨骼疾病相关。在这项研究中,我们分析了来自人类关节软骨和肋软骨(青少年和胎儿)软骨细胞通道体基因表达的一致性。值得注意的是,我们发现14个离子通道基因在关节软骨和两种类型的肋软骨软骨细胞之间普遍表达。其他离子通道基因仅在关节软骨细胞(6个基因)或肋软骨细胞(5个基因)中表达。胎儿和青少年肋软骨组织中BEST1和KCNJ2 (Kir2.1)的表达差异有统计学意义。有趣的是,大Ca(2+)激活的钾通道(BKα,或KCNMA1)在所有检查的软骨细胞中都非常高表达。在所有软骨样本的软骨细胞中也观察到Panx1、GJA1 (Cx43)和GJC1 (Cx45)的间隙连接基因的表达。总之,这些数据强调了来自不同解剖部位的细胞中软骨细胞膜通道基因表达的相似性,并可能暗示了共同的电生理信号通路是细胞控制的基础。一系列机械和代谢敏感膜通道的高表达表明,软骨细胞的机械转导可能比以前认为的更复杂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Membrane channel gene expression in human costal and articular chondrocytes.

Membrane channel gene expression in human costal and articular chondrocytes.

Membrane channel gene expression in human costal and articular chondrocytes.

Membrane channel gene expression in human costal and articular chondrocytes.

Chondrocytes are the uniquely resident cells found in all types of cartilage and key to their function is the ability to respond to mechanical loads with changes of metabolic activity. This mechanotransduction property is, in part, mediated through the activity of a range of expressed transmembrane channels; ion channels, gap junction proteins, and porins. Appropriate expression of ion channels has been shown essential for production of extracellular matrix and differential expression of transmembrane channels is correlated to musculoskeletal diseases such as osteoarthritis and Albers-Schönberg. In this study we analyzed the consistency of gene expression between channelomes of chondrocytes from human articular and costal (teenage and fetal origin) cartilages. Notably, we found 14 ion channel genes commonly expressed between articular and both types of costal cartilage chondrocytes. There were several other ion channel genes expressed only in articular (6 genes) or costal chondrocytes (5 genes). Significant differences in expression of BEST1 and KCNJ2 (Kir2.1) were observed between fetal and teenage costal cartilage. Interestingly, the large Ca(2+) activated potassium channel (BKα, or KCNMA1) was very highly expressed in all chondrocytes examined. Expression of the gap junction genes for Panx1, GJA1 (Cx43) and GJC1 (Cx45) was also observed in chondrocytes from all cartilage samples. Together, this data highlights similarities between chondrocyte membrane channel gene expressions in cells derived from different anatomical sites, and may imply that common electrophysiological signaling pathways underlie cellular control. The high expression of a range of mechanically and metabolically sensitive membrane channels suggest that chondrocyte mechanotransduction may be more complex than previously thought.

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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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